TAM与VEGF在外周T细胞淋巴瘤非特指型中表达及临床意义  

Expression and Clinical Significance of Tumor-associated Macrophages and VEGF in Peripheral T-cell Lymphoma Not Otherwise Specified

作　　者：任旭升[1] 王华庆[1] 孟祥睿[1] 钱正子[1] 周世勇[1] 张会来[1] 邱立华[1] 郝希山[1] 

机构地区：[1]天津医科大学附属肿瘤医院淋巴肿瘤科天津市肿瘤防治重点实验室中美淋巴瘤血液诊治中心,天津市300060

出　　处：《中国肿瘤临床》2012年第21期1635-1638,共4页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨肿瘤相关巨噬细胞(TAM)及血管内皮生长因子(VECF)在外周T细胞淋巴瘤非特指型(PTCL-NOS)中的表达及临床意义。方法:采用免疫组织化学方法对60例PTCL-NOS患者肿瘤组织中的CD68和VEGF进行检测,15例正常人淋巴结组织为对照。结果:肿瘤组织中CD68阳性细胞平均含量为(56.5±18.6)个/高倍镜视野,而对照组为(12.4±6.2)个/高倍镜视野(P<0.01),肿瘤组织与对照组VEGF阳性表达率分别为78.3%和26.7%(P<0.05)。TAM与骨髓侵犯、IPI评分及疗效相关(P<0.05)。TAM高表达组和低表达组的2年总生存率(overall survival,OS)分别为23.6%和55.3%(P<0.05)。VEGF的表达与肿瘤分期、骨髓侵犯和IPI评分相关(P<0.05),VEGF表达阳性组和阴性组的2年OS分别为22.9%和83.3%(P<0.01)。单变量生存分析显示VEGF表达、TAM计数、肿瘤分期、IPI评分和疗效是独立的预后影响因素(P<0.05)。多变量分析显示VEGF和疗效是独立的预后影响因素(P<0.05)。结论:TAM和VEGF在PTCL-NOS中表达明显升高,单因素分析显示二者是PTCL-NOS的不良预后因素。多因素分析显示仅VEGF是独立的预后影响因素。Objective： This work aims to detect the expression and to identify the clinical significance of tumor-associated macro- phages （TAMs） and vascular endothelial growth factor （VEGF） in peripheral T-cell lymphoma not otherwise specified （PTCL-NOS）. Methods： Immunohistochemistry was used to detect CD68 and VEGF expressions in the tumor specimens of 60 cases with PT- CL-NOS. A normal lymph node biopsy was used as the control sample. Results： The average content of the CD68-positive cells was 56.5± 18.6 per high-power field （HPF） in the PTCL-NOS tissues and 12.4±6.2 per HPF in the control sample （P〈0.01）. The VEGF-posi- tive rates for the PTCL-NOS tissues and the control sample were 78.3% and 26.7% （P〈0.05）, respectively. TAMs were significantly cor- related to bone marrow invasion, IPI score, and treatment response （P〈0.05）. The two-year overall survival （OS） was 23.6% and 55.3% in the groups with high-TAM- and low-TAM-expression, respectively （P〈0.05）. VEGF expression was closely correlated to tumor stag- ing, bone marrow invasion, and IPI score （P〈0.05）. The two-year OS was 22.9% and 83.3% in the VEGF-positive and VEGF-negative groups, respectively （P〈0.01）. A univariate survival analysis revealed that the VEGF expression, TAMs content, rumor staging, IPI score, and treatment response were the prognostic factors of patients with PTCL-NOS （/9〈0.05）. A multivariate Cox regression model showed that VEGF and treatment response were both independent OS predictors （P〈0.05）. Conclusion： TAMs and VEGF are overex- pressed in PTCL-NOS. A univariate survival analysis reveals that TAMs and VEGF are both predictive factors for PTCL-NOS prognosis, and the multivariate survival analysis using Cox regression model data show that only VEGF is an independent predictive factor for OS.

关 键 词：外周T细胞淋巴瘤 肿瘤相关巨噬细胞 血管内皮生长因子 临床意义 

分 类 号：R733.1[医药卫生—肿瘤]