白花丹素对骨肉瘤细胞系U2OS的作用及其机制  被引量:1

Effect and Mechanism of Plumbagin in Human Osteosarcoma Cell Line U2OS

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作  者:田林强[1] 陈安民[1] 尹德龙[1] 宫晨[1] 任晔[1] 郭风劲[1] 

机构地区:[1]华中科技大学同济医学院附属同济医院骨科,武汉430030

出  处:《肿瘤防治研究》2012年第11期1285-1288,共4页Cancer Research on Prevention and Treatment

基  金:973课题资助项目(2002CB513100)

摘  要:目的研究白花丹素对骨肉瘤细胞系U2OS的作用,并初步探讨其作用机制。方法用CCK-8法检测白花丹素对骨肉瘤细胞的促凋亡作用;用HOECHST33342染色研究白花丹素对骨肉瘤细胞作用后的形态学变化;用Western blot法检测白花丹素作用后骨肉瘤细胞MDM2和p53蛋白表达情况。结果 CCK-8结果显示白花丹素对骨肉瘤细胞有明显的抑制作用,而且呈浓度依赖性;HOECHST33342染色结果显示白花丹素对骨肉瘤U2OS细胞的抑制作用主要是通过促进凋亡来实现;Westernblot结果显示白花丹素能够改变p53/MDM2基因表达比例。结论白花丹素通过细胞凋亡途径抑制骨肉瘤细胞系U2OS细胞的生长。Objective To explore the effect of plumbagin on the growth of osteosarcoma cell line U2OS and its possible underlying mechanisms.Methods Cell apoptosis of plumbagin against osteosarcoma was studied by CCK-8 assay;morphological changes was studied by HOECHST 33342 staining;the expression of p53 and MDM2 gene was studied by Western blot assay.Results CCK-8 assay showed that plumbagin had an obvious inhibition on osteosarcoma cell line in a dose-dependent manner;HOECHST 33342 staining showed that plumbagin inhibited the growth of osteosarcoma cell line U2OS through the promotion of apoptosis;Western blot assay showed that plumbagin played the role by changing the expression proportion of p53 and MDM2.Conclusion Plumbagin inhibited osteosarcoma cell line U2OS cells growth through cell apoptotic pathways.

关 键 词:白花丹素 骨肉瘤细胞 凋亡 P53基因 MDM2基因 

分 类 号:R738.1[医药卫生—肿瘤] R730.52[医药卫生—临床医学]

 

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