非小细胞肺癌组织中caveolin-1和cathepsin D的表达及其与MVD的相关性  

Expressions of caveolin-1,cathepsin D and Their Correlation with MVD in Non-small Cell Lung Carcinoma

作　　者：马华玲 杨赤兵 陈卫斌 张文娟 李玲 陈洪雷[2] 朱润庆[2] 

机构地区：[1]武钢总医院病理科,武汉430080 [2]武汉大学基础医学院病理学教研室

出　　处：《肿瘤防治研究》2012年第11期1333-1336,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30900652)

摘　　要：目的探讨caveolin-1和cathepsin D蛋白在非小细胞肺癌(non-small cell lung carcinoma,NSCLC)中的表达及其与微血管密度(microvessel density,MVD)及临床病理特征之间的关系。方法用免疫组织化学法检测caveolin-1和cathepsin D在86例NSCLC和20例癌旁肺组织中的表达,以及CD34在NSCLC中的表达,并对MVD计数。结果 caveolin-1在NSCLC组织中表达率显著低于癌旁肺组织(P=0.000);而cathepsin D在NSCLC组织中表达率显著高于癌旁肺组织(P=0.024)。caveo-lin-1和cathepsin D的表达均与TNM分期(P1=0.001;P2=0.008)和淋巴结转移有关(P1=0.032;P2=0.001)。caveolin-1和cathepsin D阳性病例的MVD值明显高于阴性病例(P=0.000)。caveolin-1与cathepsin D在NSCLC中呈正相关(r=0.307;P=0.004)。结论 caveolin-1在肺癌中具有双重作用;caveolin-1、cathepsin D高表达可能刺激间质内微血管密度增高,共同促进肺癌的侵袭和转移。Objective To study the expression of caveolin-1 and cathepsin D in non-small cell lung carcinoma（NSCLC） and their correlation with microvessel density（MVD） and clinicopathologic parameters.Methods Immunohistochemistry staining was used to detect caveolin-1,cathepsin D expression in 68 NSCLCs and 20 adjacent noncancerous lung tissues and CD34 expression in NSCLC.MVD was detected by CD34 immunostaining.Results The positive rate of caveolin-1 protein in NSCLC was significantly lower than those in adjacent noncancerous lung tissues（P=0.000）.On the contrary,the positive rate of cathepsin D in NSCLC was significantly higher than those in adjacent noncancerous lung tissues（P=0.024）.Caveolin-1 and cathepsin D expression were both correlated with higher TNM stage（P1=0.001;P2=0.008） and lymph node metastasis（P1=0.032;P2=0.001）.MVD in the positive expression of caveolin-1 and cathepsin D were higher than those in the negative cases（P=0.000）.The expression caveolin-1 was positively correlated with the expression of cathepsin D in the NSCLC（r=0.307;P=0.004）.Conclusion caveolin-1 may have a dual effect in the NSCLC.High expression of caveolin-1 and cathepsin D may play a vital role in the progression of NSCLC and closely correlate with tumor angiogenesis.

关 键 词：肺肿瘤 CAVEOLIN-1 CATHEPSIND 微血管密度 

分 类 号：R734.2[医药卫生—肿瘤]