EDTA凝胶和氯亚明对人牙周膜细胞增殖和碱性磷酸酶活性的影响  

Effect of Chloramine-T and EDTA Gel on the Proliferation and Activity of Alkaline Phosphatase of the Periodontal Ligament Cell

作　　者：赵燕申[1] 夏文薇[1] 

机构地区：[1]上海市口腔医学研究所.上海市口腔医学重点实验室,上海200011

出　　处：《口腔医学研究》2012年第11期1138-1140,共3页Journal of Oral Science Research

基　　金：上海市科学技术委员会基金资助(编号:10JC1408800)

摘　　要：目的:探讨EDTA凝胶(EDTA gel)和氯亚明(chloramine-T)对人牙周膜细胞(periodontal ligament cell,PDLC)增殖和碱性磷酸酶(alkaline phosphatase,ALP)活性的影响。方法:将5种不同浓度的EDTA凝胶和氯亚明溶液分别作用于体外培养的PDLC。MTT法观察PDLC的增殖情况,酶动力学方法检测PDLC的碱性磷酸酶活性。结果:浓度为0.005%、0.0025%和0.00125%的EDTA凝胶溶液及浓度为0.0025%和0.00125%的氯亚明对PDLC的增殖和ALP活性无抑制作用。浓度为0.02%和0.01%的EDTA凝胶溶液及浓度为0.02%、0.01%和0.005%的氯亚明对PDLC的增殖和ALP活性有抑制作用(P<0.05),抑制作用随药物浓度和作用时间的增加而增加。结论:EDTA凝胶和氯亚明对PDLC的增殖和碱性磷酸酶活性具有一定的抑制作用。Objective： To examine the effect of chloramine--T and EDTA gel on the proliferation and the alkaline phosphatase （ALP） activity of the periodontal ligament cell （PDLC）. Methods： We have added five different con- centrations of chloramine--T and EDTA gel on the PDLC in vitro. Then we observed the proliferation of PDLC by MTT and detected ALP activity of PDLC by enzyme dynamics. Results： When the concentrati6ns of EDTA gel solu- tion were 0. 005~, 0. 0025~/6o and 0. 00125~ and the concentrations of chloramine-- T were 0. 0025~//00 and 0. 00125 ~, the proliferation and ALP activity of PDLC was not inhibited. When the concentrations of EDTA gel solu- tion were 0.02~ and 0.01~ and the concentrations of chloramine--T were 0.02%, 0.01%and 0. 005%, the pro- liferation and ALP activity of PDLC was inhibited （P〈0.05）. The effects of inhibition increase as the action time and concentration of drugs. Conclusion： EDTA gel and chloramine--T can inhihit the proliferation and ALP activity of PDLC to a certain extent.

关 键 词：牙周膜细胞 EDTA凝胶 氯亚明 MTT 碱性磷酸酶 

分 类 号：R781.05[医药卫生—口腔医学]