电化学法研究多粘菌素B与磷脂酰丝氨酸的相互作用及与其他磷脂的比较  

Electrochemical Analysis on the Interaction of Polymyxin B with Phosphatidylserine and Comparison to Other Phospholipids

作　　者：屈凌波[1,2] 冉瑜[1] 白希希[1] 牛真真[1] 于岚岚[1] 

机构地区：[1]郑州大学化学系,河南郑州450001 [2]河南工业大学化学化工学院,河南郑州450052

出　　处：《分析测试学报》2012年第11期1352-1357,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金(21002093);教育部留学回国人员科研启动基金;中国博士后科学基金资助项目

摘　　要：采用头基修饰的磷脂在金电极表面构建了稳定的磷脂双层膜,并使用该膜模拟生物膜对多肽类抗生素多粘菌素B(PMB)和磷脂酰丝氨酸的相互作用进行了研究。PMB可与磷脂酰丝氨酸发生相互作用,破坏双层膜结构,从而使膜的通透性升高。PMB的浓度、作用时间以及膜中胆固醇的存在均影响二者的作用程度。被PMB破坏的双层膜电极在一定程度上可在KCl溶液中重新自组装,且自修复的程度与修复时间和PMB的浓度有关。此外,比较了PMB和多种磷脂之间的作用程度,磷脂的头基、烃链的长度以及不饱和度均会影响二者间的相互作用。A stable phospholipid bilayer membrane was constructed on Au electrode surface by using a headgroup modified phospholipid and applied as a biomembrane model to investigate the interaction of an antibiotic polymyxin B with phosphatidylserine. Polymyxin B interacted with phosphatidylserine and destroyed the bilayer structure, resulting in increasing membrane permeation. The extent of interaction was dependent on polymyxin B concentration, reaction time and presence of cholesterol. The destroyed bilayer membrane could undergo self-recovery to some extent in KCl solution and the self-recovery was dependent on recovery time and concentration of polymyxin B. In addition, the interactions between PMB and different phospholipids were compared. Headgroup, alkyl chain length and saturation of phospholipid all affected the interaction.

关 键 词：多粘菌素B 磷脂双层膜 磷脂酰丝氨酸 循环伏安法 相互作用 

分 类 号：O657.1[理学—分析化学] S859.796[理学—化学]