菊花上2种病毒的检测及其部分外壳蛋白基因序列分析  

Detection and sequence analysis of partial coat protein gene from two viruses in chrysanthemum

作　　者：雷强[1,2] 李旻[1] 丁元明[1] 王云月[2] 

机构地区：[1]云南出入境检验检疫局技术中心,昆明650228 [2]云南农业大学植物保护学院,昆明650201

出　　处：《华中农业大学学报》2012年第6期704-709,共6页Journal of Huazhong Agricultural University

基　　金：云南省社会发展科技计划项目(2010CD143)

摘　　要：采集昆明地区花卉基地和公园的269份菊花样品,调查并分析侵染菊花的番茄不孕病毒(Tomatoaspermy virus,TAV)和菊花B病毒(Chrysanthemum virus B,CVB)的发病状况。ELISA和RT-PCR检测结果表明,TAV和CVB在菊花植株上的发病率分别为4.62%和16.70%。对3个TAV分离物和8个CVB分离物的外壳蛋白(coat protein,CP)基因的部分序列进行测定,并分别与GenBank登录的TAV和CVB序列进行比对和系统进化分析,结果显示各TAV分离物的CP基因核苷酸序列同源性为85.04%～98.02%。在系统进化树中聚集的2个簇中,昆明分离物都在同一簇。CVB的CP基因存在较明显的序列变异,核苷酸序列同源性为75.09%～84.99%,在系统进化树中聚为3个簇,昆明分离物均集中在同一簇。Tomoto aspermy virus（TAV） and Chrysanthemum virus B（CVB） were detected after samples of chrysanthemum showing noticeable symptom of virus diseaseas were collected at flower bases and parks in Kunming in 2011.On the basis of ELISA test and RT-PCR detection,the resulted incidences of the TAV and CVB were 4.62% and 16.70% respectively.Partial coat protein（CP） gene in 3 isolates of TAV and 8 isolates of CVB were sequeced.The sequencing results indicate the homology between the 3 TAV isolates in Kunming and other 28 accessed isolates in GenBank is 85.04%-98.02%.These isolates were built up in two clusters in the phylogenetic tree,and the TAV isolates in Kunming were built up in the same cluster.However,a comparison between the 8 isolates of CVB in Kunming and the other known 35 isolates in GenBank indicates their homology is 75.09%-84.99%.These isolates were built up in three clusters in the phylogenetic tree,and the CVB isolates in Kunming were built up in the same cluster.

关 键 词：菊花 番茄不孕病毒 菊花B病毒 外壳蛋白基因 系统进化分析 

分 类 号：S432.41[农业科学—植物病理学] S682.11[农业科学—农业昆虫与害虫防治]