硒和钙对蛹虫草活性物质含量的影响  

Effects of Selenium and Calcium on the Content of Cordyceps militaris Active Substances

作　　者：翁梁[1] 温鲁[2] 

机构地区：[1]江苏食品职业技术学院食品工程系,江苏淮安223003 [2]淮阴师范学院资源微生物研究所,江苏淮安223300

出　　处：《北方园艺》2012年第22期159-161,共3页Northern Horticulture

摘　　要：向培养液中分别添加不同浓度的亚硒酸钠、氯化钙,用液体振荡培养法培养蛹虫草菌丝体,用HPLC法测定虫草素与腺苷含量,用苯酚-硫酸法测定多糖含量,用高碘酸钠比色法测定虫草酸含量。以研究2种微量元素对蛹虫草菌丝体中虫草素、腺苷、多糖和虫草酸含量的影响。结果表明:向培养液中添加适量的硒或钙,可显著提高蛹虫活性物质含量。添加15mg/L亚硒酸钠,虫草素和虫草酸含量比对照组分别提高了29.0%和34.9%;添加16mg/L氯化钙,虫草素含量比对照提高24.05%。Adding different levels of Na2SeO3and CaCl2to the culture medium,with the method of liquid shake culture,the effect of 2trace elements on the content of cordycepin and adenosine,polysaccharide and cordyceps acid were studied,with HPLC to test the level of cordycepin and adenosine;with phenol-sulphate acid to test the level of polysaccharide;with sodium periodate colorimetric to test the level of cordyceps acid.The results showed that adding suitable selenium or calcium could obviously raised the content of Cordyceps militaris active substances.Adding 15mg / L Na 2 SeO 3 to the culture medium and the level of cordycepin and cordyceps acid had raised 29.0% and 34.9% respectively.Adding 16mg / L CaCl 2 and the level of cordycepin had raised 24.05%.

关 键 词：蛹虫草 亚硒酸钠 氯化钙 液体振荡培养 活性物质 

分 类 号：S567.39[农业科学—中草药栽培]