分散液液微萃取-气相色谱-质谱分析尿样中的氯氮卓  

Analysis of Chlordiazepoxide in urine by dispersive liquid-liquid micro-extraction and gas chromatography mass spectrometry

作　　者：李谈[1] 周娜[1] 梁玲琳[1] 周海梅[1] 齐孝蕊[1] 

机构地区：[1]河南科技大学法医学院,河南洛阳471003

出　　处：《福建分析测试》2012年第5期38-40,共3页Fujian Analysis & Testing

基　　金：河南科技大学SRTP项目(编号:2010078)

摘　　要：目的:用分散液液微萃取-气相色谱-质谱联用技术提取检测生物检材中的氯氮卓。方法:以氯仿为微萃取剂,甲醇为分散剂,在pH8提取尿样中的氯氮卓,气相色谱-质谱法分析,用内标标准曲线法测定氯氮卓的含量。结果:尿样中氯氮卓的线性范围为0.01～2μg/ml,检出限为0.002?g/ml;方法回收率为87.3%,RSD=3.47%(n=5)。结论:该方法操作简便、灵敏度高,适于尿样中氯氮卓的快速分析。Objective： To detect Chlordiazepoxide in urine by dispersive liquid-liquid micro-extraction and gas chromatography mass spectrometry. Methods： Chlordiazepoxide in urine was extracted with chloroform and methanol at pH8 and was analyzed by GC-MS, its content was determined with internal standard curve. Results： The detection limit of the Chlordiazepoxide in urine was 0.002μ g/ml, and the standard curve was linear in the range of 0.01 -2 μ g/ml. The average recovery of Chlordiazepoxide was 89.8% （RSD = 3.86%, n = 5 ）. Conclusion： This method is simple, high sensitivity and suitable for quick analysis of the Chlordiazepoxide in urine.

关 键 词：氯氮卓 分散液相微萃取 气相色谱质谱联用 尿 

分 类 号：R927.2[医药卫生—药学]