miR-449a/b在Ⅱ型子宫内膜癌中表达下调  

Down-regulation of miR-449a/b in Type Ⅱ Endometrial Carcinoma

作　　者：薛纪森[1] 张乾[1] 李晓琳[1] 胡燕[1] 杨孝军[1] 颜笑健[1] 郑飞云[1] 

机构地区：[1]温州医学院附属第一医院妇科,325000

出　　处：《医学研究杂志》2012年第11期103-107,共5页Journal of Medical Research

基　　金：浙江省自然科学基金资助项目(Y2090699)

摘　　要：目的分析Ⅰ型和Ⅱ型子宫内膜癌及良性子宫内膜组织间MicroRNA(miRNA)表达谱差异。方法收集子宫内膜癌手术患者内膜及良性患者内膜组织,利用免疫组化法分组,分别提取总RNA,基因芯片检测差异表达的miRNA,并经反转录PCR和荧光定量PCR验证差异表达的miRNA。结果芯片检测发现Ⅱ型子宫内膜癌与Ⅰ型子宫内膜癌及良性内膜组织间存在显著下调的miRNA分别有12个和9个,均下调的有miR-449a/b和miR-20b,显著上调的miRNA分别有18个和4个,均上调的有miR-29a。经反转录PCR和荧光定量PCR验证后发现miR-449a在Ⅱ型子宫内膜癌比Ⅰ型子宫内膜癌及良性内膜组织分别下调347倍和288倍,miR-449b分别下调461倍和424倍。结论Ⅰ型和Ⅱ型子宫内膜癌间miRNA的表达差异可能与不同类型子宫内膜癌的发生发展相关,而miR-449a/b可能与Ⅱ型子宫内膜癌的发病机制有关,可为进一步研究子宫内膜癌发生的分子机制提供依据。Objective To study microRNA expression patterns in type Ⅰ and Ⅱ endometrial carcinoma （EC） and benign endometri- urn. Methods Fresh - frozen samples of endometrial carcinoma and benign endometrial tissues were collected and divided into three groups by immunohistochemistry. Total RNA was extracted from samples and miRNA expression profiles were evaluated by gene arrays. Dysregulated miRNAs were verified by reverse transcription - PCR and real - time quantitative reverse transcription - polymerase chain re- action （qRT- PCR）. Results Gene arrays identified that 12 and 9 miRNAs were significantly down -regulated, while 18 and 4 were significantly up - regulated, both in type Ⅱ ECs when compared to type Ⅰ ECs or benign endometrium respectively. Among these miRNAs, miR -449a/b were both down - regulated and were selected for verification by qRT - PCR. We found that miR -449a showed down - reg- ulation for about 347 - fold and 288 - fold, miR -449b showed 461 - fold and 424 - fold in type Ⅱ ECs, when compared to type Ⅰ ECs and benign endometrium respectively. Conclusion Different expression profiles of miRNA between type Ⅰ and Ⅱ ECs may provide insights into their roles in the pathogenesis and progression of EC. Based on this study,miR -449a/b is predicted to be involved in tumorigenesis and tumor progression in type Ⅱ EC. The study of miRNA provides a framework on which further research into novel diagnosis and treat- ment of EC can be focused.

关 键 词：子宫内膜癌 MICRORNA miR-449a b 基因芯片 荧光定量PCR 

分 类 号：R737.33[医药卫生—肿瘤]