猪源乙型脑炎病毒河南分离株的全基因组测序及进化分析  

Full-length Genomes and Phylogenetic Analysis of Three Japanese Encephalitis Virus Isolates from Swine in Henan

作　　者：禹乐乐[1,2] 滕蔓[2] 罗俊[2,3] 迟佳琦[2] 余祖华[2,3] 柴书军[2] 梁晓晓[2] 张改平[2] 王爱萍[1] 

机构地区：[1]郑州大学生物工程系,河南郑州450001 [2]河南省农业科学院农业部动物免疫学重点开放实验室河南省动物免疫学重点实验室,河南郑州450002 [3]河南科技大学动物科技学院,河南洛阳471003

出　　处：《华北农学报》2012年第5期184-190,共7页Acta Agriculturae Boreali-Sinica

基　　金：国家"公益性"行业(农业)科研专项经费资助(201203082-5)

摘　　要：为了解猪源乙型脑炎病毒(JEV)的全基因组结构及其分子进化规律,测定了3个JEV猪源分离株(BSF.ZZ-1、BSF.ZZ-3和CSF.XZ-2D)的全基因组序列,并对它们的氨基酸序列进行了分析。结果表明,CSF.XZ-2D全长为10 976 nt,而BSF.ZZ-1和BSF.ZZ-3全长均为10 977 nt,在后2个毒株基因组的10 701 nt位点处均有1个插入碱基"G"。BSF.ZZ-1、BSF.ZZ-3、CSF.XZ-2D与SA14株核苷酸和氨基酸同源性较高,核苷酸同源性都为99.4%,氨基酸同源性均为99.0%,与猪源分离株HW(HW1)、HWe(HW2)和SH0601的核苷酸与氨基酸同源性均为98.0%以上。用涵盖全部5个基因型的30个JEV毒株的全基因组序列构建系统进化树,发现这3个猪源分离株位于同一进化分支,均属于基因Ⅲ型。In order to learn more about the complete genome and molecular evolution regular of Japanese encephalitis virus（JEV） strains isolated from swine,the full-length genome sequences of three JEV isolates,namely as BSF.ZZ-1,BSF.ZZ-3 and CSF.XZ-2D isolated from swine were analyzed in this paper.The results show that the length of the complete genomes of BSF.ZZ-1,BSF.ZZ-3 and CSF.XZ-2D was 10 977,10 977,10 976 nt,respectively.There is an insert base of ＂G＂ at the 10 701 nt site in the genomes of both BSF.ZZ-1 and BSF.ZZ-3.Compared to the virulent strain SA14,the homologies of nucleotide and amino acid sequences of BSF.ZZ-1,CSF.XZ-2D and BSF.ZZ-3 are 99.4% and 99.0%,respectively.Compared to the isolates HW（HW1）,HWe（HW2） and SH0601 from swine,the homologies of the present three isolates are all more than 98.0%.Phylogenetic analysises with other 30 reference isolates covering all five JEV genotypes indicated that BSF.ZZ-1,CSF.XZ-2D and BSF.ZZ-3 located in a same evolutionary branch,belonging to the genotype III.

关 键 词：猪 乙脑病毒 全基因组 系统进化分析 

分 类 号：Q78[生物学—分子生物学] S436[农业科学—农业昆虫与害虫防治]