近红外荧光量子点表皮生长因子受体单克隆抗体探针对头颈部鳞状细胞癌活体可视化成像和体内分布的动物实验研究  

In situ visual imaging and in vivo distribution of head and neck squamous cell carcinoma in mice by near-infrared fluorescent quantum dots epidermal growth factor receptor monoclonal antibody probe

作　　者：唐洪[1] 杨凯[1] 赵成[1] 白云龙[1] 黄昊[1] 

机构地区：[1]重庆医科大学附属第一医院口腔颌面外科,重庆400016

出　　处：《华西口腔医学杂志》2012年第6期568-573,共6页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(81172205)

摘　　要：目的探讨近红外荧光量子点(QDs)表皮生长因子受体(EGFR)单克隆抗体(mAb)探针对头颈部鳞状细胞癌的原位可视化成像和体内分布情况。方法将发射波长为800 nm的近红外荧光QDs与EGFR mAb连接,制备QD800-EGFR mAb探针。在体外将QD800-EGFR mAb与人颊鳞状细胞癌BcaCD885细胞共培养30 min,使用激光扫描共聚焦显微镜(LSCM)观察QD800-EGFR mAb对BcaCD885细胞的结合情况。将QD800-EGFR mAb通过尾静脉注射到裸鼠头颈部鳞状细胞癌模型,在不同时间点通过活体成像观察QD800-EGFR mAb对头颈部鳞状细胞癌的可视化成像情况和QD800-EGFR mAb在体内的分布。结果静脉注射QD800-EGFR mAb探针后能对裸鼠头颈部鳞状细胞癌进行清楚的可视化荧光成像,成像一直持续到24 h,但在30 min～6 h时间段内肿瘤成像最完整和荧光信噪比最高。对体内QD800-EGFR mAb在肿瘤和器官的分布检测证明:QD800在肝中分布最多,在肿瘤中的聚集随着时间的延长逐渐下降,心、脑、肠、肺、胃中均未见有QD800。结论 QD800-EGFR mAb探针对头颈部癌能进行清楚的可视化个体成像检测,在非侵入可视化成像研究头颈部癌的发生发展和个体化治疗等方面有巨大的发展前景。Objective To investigate the in situ visualization imaging and the in vivo distribution of the near- infrared fluorescent quantum dots （QDs） epidermal growth factor receptor（EGFR） monoclonal antibody （mAb） probe in head and neck sqnamous cell carcinoma （HNSCC）. Methods QDs with an emission wavelength of 800 nm （QDS00） was conjugated with EGFR mAb to produce QDS00-EGFR mAb. QDS00-EGFR mAb was co-cultured with BcaCD885 squamous cancer cell line for 30 min and observed with laser scanning confocal microscope（LSCM）. QDS00-EGFR mAb was injected into HNSCC animal model through the tail vein, and the in situ visualization imaging and the in vivo distribution of QDS00-EGFR mAb was analyzed at different time points. Results BcaCD885 squamous cell carci- noma in the head and neck can be imaged clearly and visually after intravenous injection of QDS00-EGFR mAb probe, these fluorescence signals lasted for 24 h. The most complete tumor images with maximal signal-to-noise ratio were observed from 30 min to 6 h after injection of the probe. In vivo tissue distribution studies demonstrated that QD800 aggregated mostly in liver. QDS00 aggregation decreased with time in tumors, and QDS00 didn＇t aggregate in heart, brain, intestine, lung and stomach. Conclusion The QDS00-EGFR mAb probe can clearly produce visual images in head and neck cancer. It has promising prospects in visualization in vivo imaging and individual treatment of head and neck cancer.

关 键 词：头颈癌 量子点 表皮生长因子受体 成像 体内分布 

分 类 号：R739.91[医药卫生—肿瘤]