内皮细胞型一氧化氮合成酶Glu298Asp基因多态性与牙周炎伴Ⅱ型糖尿病的相关性研究  

Relationship between endothelial nitric oxide synthase Glu298Asp gene polymorphisms and the chronic periodontitis with type 2 diabetes mellitus

作　　者：侯海娟[1,2] 张帆[3] 赵美林[1,2] 曹桂芬[4] 黄海云[5] 黄萍[5] 

机构地区：[1]重庆医科大学附属口腔医院牙周科 [2]重庆市口腔疾病与生物医学研究中心,重庆401147 [3]四川大学华西医院体检中心,成都610041 [4]浙江大学医学院附属口腔医院牙周科,杭州310006 [5]四川大学华西口腔医院牙周科,成都610041

出　　处：《华西口腔医学杂志》2012年第6期628-631,共4页West China Journal of Stomatology

基　　金："十一五"国家科技攻关计划基金资助项目(2007BAI-18B02)

摘　　要：目的研究内皮细胞型一氧化氮合成酶(eNOS)Glu298Asp基因多态性与牙周炎伴Ⅱ型糖尿病易感性的关系。方法收集慢性牙周炎患者、慢性牙周炎伴Ⅱ型糖尿病患者、Ⅱ型糖尿病患者和牙周健康者的颊黏膜拭子,提取DNA后应用聚合酶链反应-限制性片段长度多态性法检测eNOS Glu298Asp的基因型分布。结果慢性牙周炎组、Ⅱ型糖尿病组、慢性牙周炎伴Ⅱ型糖尿病组、正常组eNOS Glu298Asp基因型的分布差异有统计学意义(掊2=18.503,P=0.005),等位基因频率分布差异也有统计学意义(掊2=8.243,P=0.041)。慢性牙周炎伴Ⅱ型糖尿病组与正常组相比,T基因型的OR值为0.962,95%可信区间为0.737～1.256,说明T基因型可能为糖尿病和牙周炎的保护因素;G基因型的OR值为1.043,95%可信区间为0.781～1.391,说明G基因型可能为二者的危险因素,但差异无统计学意义。结论慢性牙周炎、慢性牙周炎伴Ⅱ型糖尿病、糖尿病的易感性可能与eNOS Glu298Asp多态性有关。Objective To study the relationship between endothelial nitric oxide synthase （eNOS） Glu298Asp gene polymorphism and chronic periodontitis（CP） with type 2 diabetes mellitus（T2DM）. Methods DNA from patients＇ buc- cal swabs of CP, CP with T＇2DM, T2DM and health was isolated and extracted. The eNOS Glu298Asp gene polymor- phism were assessed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） respectively. Results The distribution of eNOS Glu298Asp genotype in CP group, T2DM group, CP with T2DM group and health group showed statistical differences （Xz=18.503, P=0.005）, and the gene frequency showed statistical differences （X2= 8.243, P--O.041）. Compared health group to CP with T2DM group, OR value of the genetype T was 0.962, 95% con- fidence interval lay in between 0.737 to 1.256, which showed T maybe a protective factor. While OR value of the genetype G was 1.043, 95% confidence interval lay in between 0.781 to 1.391, which showed G maybe a risk factor. However, neither T nor G was statistically significant. Conclusion Based on these findings, there are some associa- tion between eNOS Glu298Asp polymorphism and the risk of CP group, T2DM group, CP with T2DM group.

关 键 词：慢性牙周炎 Ⅱ型糖尿病 一氧化氮合成酶 基因多态性 

分 类 号：R781.4[医药卫生—口腔医学] R587.1[医药卫生—临床医学]