富血小板纤维蛋白与富血小板血浆体外释放生长因子的比较及其对脂肪干细胞增殖分化的影响  

The comparison of platelet-rich fibrin and platelet-rich plasma in releasing of growth factors and their effects on the proliferation and differentiation of adipose tissue-derived stem cells in vitro

作　　者：杨世茂[1,2] 王明国[2] 李静[3] 刘金盼[3] 林夏莲[3] 徐炜[3] 

机构地区：[1]山东大学口腔医院口腔颌面外科,济南250012 [2]济南市中心医院口腔科,济南250013 [3]潍坊医学院口腔医学院,潍坊261053

出　　处：《华西口腔医学杂志》2012年第6期641-644,649,共5页West China Journal of Stomatology

摘　　要：目的比较富血小板纤维蛋白(PRF)与富血小板血浆(PRP)体外释放生长因子的质量浓度及其对脂肪干细胞(ADSCs)增殖和成骨分化的影响。方法抽取兔耳中央动脉血,一次离心法制备PRF,二次离心法制备PRP,分别将其置于5 mL新鲜的α-MEM培养液中,分别于37℃下静置1、7、14、21、28 d,收集PRF与PRP析出液,检测析出液中转化生长因子-β1(TGF-β1)及血小板源性生长因子-AB(PDGF-AB)的质量浓度。将收集的PRF与PRP析出液配置成条件培养液培养ADSCs,观察其对ADSCs增殖及碱性磷酸酶(ALP)活性的影响。结果 1)生长因子释放情况:不同时间点的PRF析出液中,14 d时TGF-β1的质量浓度达到最高,7 d时PDGF-AB的质量浓度最高;不同时间点的PRP析出液中,1 d时TGF-β1与PDGF-AB的质量浓度即达最高,以后逐渐下降。2)对ADSCs增殖及ALP活性的影响:PRF析出液中,14 d时对其影响最大;PRP析出液中,1 d时对其影响最大。结论与PRP相比,PRF能够缓慢持久地释放生长因子,更有力地刺激ADSCs的增殖和分化。Objective To compare the releasing of growth factors between platelet-rich fibrin （PRF） and platelet- rich plasma （PRP） as well as their effects on the proliferation and differentiation of adipose tissue-derived stem cells （ADSCs） in vitro. Methods Blood was taken from central artery of rabbits, acquiring PRF was acquired through one time centrifuge and PRP through two times centrifuge. Five milliliters of fresh ct-MEM was added to PRF and PRP and incubated at 37 %. The time points to collect exudates was in day 1, 7, 14, 21, 28 and the mass concentrations of transforming growth factor-151 （TGF-151） and platelet derived growth factor-AB（PDGF-AB） were quantified in PRF and PRP. Then the exudates of PRF and PRP were used to culture ADSCs and evaluate the effects of PRF and PRP on proliferation and differentiation of ADSCs. Results 1）Growth factor release： In the PRF exudates at different time points, the mass concentration of TGF-~I was the highest at day 14 and the highest mass concentration of PDGF-AB at day 7, the mass concentration of both TGF-131 and PDGF-AB was the highest at the first day and then gradually declined. 2）The effect on proliferation and differentiation： PRF exudates of day 14 expressed the maximum proliferation and alkaline phosphatase （ALP） activity. PRF exudates of day 1 demonstrat the maximum proliferation and ALP activity. Conclusion Comparing to PRP, PRF releases growth factors gradually and expressed stronger and more durable effect on proliferation and differentiation of ADSCs in vitro.

关 键 词：富血小板纤维蛋白 富血小板血浆 脂肪干细胞 成骨分化 

分 类 号：R783[医药卫生—口腔医学]