氧化苦参碱对人胃癌细胞BGC-823生长活性和环氧合酶-2表达的影响  

The effect of oxymatrine on cell proliferation and COX-2 expression in human gastric cancer cell line BGC-823

作　　者：杨伟 摆茹[2] 

机构地区：[1]宁夏自治区第三人民医院消化内科,宁夏银川750021 [2]宁夏医科大学基础医学院,宁夏银川750004

出　　处：《宁夏医学杂志》2012年第11期1076-1078,共3页Ningxia Medical Journal

摘　　要：目的观察氧化苦参碱(OM)对人胃癌细胞BGC-823生长活性和环氧合酶(COX)-2表达的影响。方法体外培养人胃癌细胞BGC-823,采用MTT法检测不同浓度和作用时间下OM对细胞生长活性的影响;Western Blot和Real-time RT-PCR检测COX-2蛋白和mRNA的表达水平。结果与对照组比较,低浓度(1.0mg/ml)OM处理组BGC-823细胞的生长活性未受到抑制,中浓度(2.0 mg/ml)和高浓度(4.0 mg/ml)OM处理组细胞的生长活性受到明显抑制(P<0.05),且抑制效应随药物剂量和作用时间的增长而增强;同时伴有COX-2蛋白和mRNA表达水平降低(P<0.05)。结论 OM具有抑制人胃癌细胞BGC-823生长活性的作用,其作用机制可能与下调COX-2的表达相关。Objective To observe the effect of oxymatrine （OM） on cell proliferation and COX -2 expression in human gastric cancer cell line BGC - 823. Methods Human gastric cancer BGC - 823 cells was cultured in vitro with oxymatrine, the MTT assay was used to determine cell viability at 24h ,48 h and 72h. Western Blot and Real - time RT - PCR were applied to examine the protein and mRNA expression of COX - 2. Results Low - dose （ l. 0 mg/mL） oxymatrine had no inhibitory effect on cell viability of BGC - 823 cells. Oxymatrine significantly inhibited cell viability in a time and concentration - dependent manner when concentration exceeded 1 mg/ ml （ P 〈 0.05 ）, and down - regulated the protein and mRNA expression of COX - 2 （ P 〈 0.05 ）. Conclusion Within a certain drug concentration, oxymatrine can inhibit cell viability of BGC - 823 cells and down - regulating the expression of COX - 2.

关 键 词：氧化苦参碱 胃癌 生长活性 环氧合酶 

分 类 号：R735.2[医药卫生—肿瘤]