气滞血瘀证大鼠H_2S/CSE、CBS体系的变化  

The change H_2S/CSE、CBS in Qi stagnation and blood stasis

作　　者：宁天一[1] 王婷婷[1] 程嘉艺[1] 

机构地区：[1]辽宁中医药大学,沈阳110032

出　　处：《中药药理与临床》2012年第5期234-237,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：辽宁省自然科学基金项目;20102143

摘　　要：目的:观察气滞血瘀证模型大鼠体内硫化氢/胱硫醚-γ-裂解酶(H2S/CSE)、硫化氢/胱硫醚-β-合成酶(H2S/CBS)变化。方法:将Wistar大鼠随机分成4组:对照组、模型组、血府逐瘀汤低剂量组(34.5g/kg)、血府逐瘀汤组高剂量组(103g/kg)。采用多因素联合刺激法建立气滞血瘀模型。运用分光光度法测定血浆、肝组织中H2S含量,利用RT-PCR法测定肝组织中CSE、CBS基因表达。采用免疫组化法检测颈总动脉平滑肌细胞中CSE表达情况。结果:模型组血浆H2S含量(42.64±1.67)μmol/L显著低于对照组(105.52±3.07)μmol/L(P<0.01),血府逐瘀汤低剂量(34.5g/kg)血浆H2S含量(79.34±0.57)μmol/L显著高于模型组(42.64±1.67)μmol/L(P<0.01);模型组肝组织中H2S含量(44.54±2.33)μmol/L显著低于对照组(70.35±2.47)μmol/L(P<0.01),血府逐瘀汤组低剂量组肝组织中H2S含量(63.99±4.53)μmol/L显著高于模型组(44.54±2.33)μmol/L(P<0.01);模型组肝组织CSE表达水平显著低于对照组(P<0.01),血府逐瘀汤组低剂量组肝组织CSE表达水平显著高于模型组(P<0.05),肝组织中的CBS表达变化没有统计学意义(P>0.05)。模型组CSE蛋白表达量显著低于对照组(P<0.01),血府逐瘀汤组低剂量组CSE蛋白表达量显著高于模型组(P<0.05)。结论:采用多因素联合刺激法建立的气滞血瘀证大鼠中H2S/CSE体系下调。Objective：To investigate plasma hydrogen sulfide（H2S） levels and cystathionine-γ-lyase（CSE） and cystathionine-β-synthase（CBS） mRNA expression in the liver tissues in Qi stagnation and blood stasis rats and to explore the roles of endogenous H2S/CSE and H2S/CBS system in the pathogenesis of Qi stagnation and blood stasis.Methods：Wistar rats were randomly divided into four groups：normal control group model group low concentration and high concentration.The Qi stagnation and blood stasis model of rats were established by complex pathogens.The content of H2S in plasma and liver tissues of rats was measured by spectrophotometric.RT-PCR was used to analyze liver tissues CSE and CBS mRNA expression.Results：The plasma H2S of the model group（42.64±1.66）μmol/L was decreased significantly compared with normal control group（105.52±3.07）μmol/L（P0.01）.The plasma H2S of treatment group（79.34±0.57）μmol/L was increased significantly compared with model group（42.64±1.67）μmol/L（P0.01）.The H2S in liver tissues of model group（44.54±2.33）μmol/L was decreased significantly compared with normal control group（70.35±2.47）μmol/L（P0.01）.The H2S in liver tissues of treatment group（63.99±4.53）μmol/L was increased significantly compared with model group（44.54±2.33）μmol/L（P0.01）.CSE mRNA expression in the model group were significantly lower than normal control group（P0.01）.CSE mRNA expression in the treatment group were significantly increased than model group（P0.05）.The contents of CBS mRNA expression in the model group were not statistically different from those in the control and low concentration.CSE protein expression in the model group were significantly lower than normal control group（P0.01）.CSE protein expression in the treatment group were significantly increased than model group（P0.05）.Conclusions：H2S/CSE systems appear to have some effect on liver tissues in Qi stagnation and blood stasis rats.

关 键 词：气滞血瘀 硫化氢 胱硫醚-Γ-裂解酶 胱硫醚-Β-合成酶 

分 类 号：R-332[医药卫生]