DAPI联合PKH26标记骨髓干细胞在急性肝脏损伤模型小鼠肝内迁移的示踪作用  

The tracing effect of BMMSC double-labeled by DAPI combined PKH26 in acute liver failure model of mice

作　　者：金世柱[1] 韩明子[1] 刘冰熔[1] 曲波[1] 宋吉涛[1] 褚艳杰[1] 赵瑞波[2] 黄褀 班翔[2] 

机构地区：[1]哈尔滨医科大学附属第二医院消化内科,哈尔滨150086 [2]哈尔滨医科大学基础医学院病理学教研室

出　　处：《胃肠病学和肝病学杂志》2012年第11期1043-1046,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨PKH26和DAPI联合标记骨髓干细胞在肝脏组织内迁移过程中的示踪作用。方法用PKH26和DAPI联合标记从Balb/c小鼠骨髓中分离出骨髓干细胞,用CCl4腹腔注射的方法制备小鼠急性肝脏损伤模型后,将标记的BMMC经尾静脉移植入急性肝脏损伤模型小鼠体内,对照组经尾静脉注入磷酸盐缓冲液(PBS)。移植2周后取肝脏组织,通过激光共聚焦荧光显微镜观察骨髓干细胞向肝脏内迁移的情况。结果接受骨髓干细胞移植组小鼠肝脏组织内可发现DAPI和PKH26双重标记的骨髓干细胞。结论 PKH26和DAPI可以联合标记向急性肝损伤模型肝组织迁移的骨髓干细胞,为体内示踪骨髓干细胞脑内迁移提供更加准确的实验方法。Objective To study the tracing effect of bone marrow stem cells double-labeled by DAPI combined PKH26 in mice model of acute liver failure induced by CC14 administration. Methods BMSCs were harvested from BALB/c mice and then double-labeled by PKH26 and DAPI. The labeled cells were subsequently infused into the tail veins of mice in which acute liver injured model had been induced by CC14 administration. 14 days after transplantation, the migration status of the cells was studied by fluorescence microscopy. Results Positive cells pre-labeled by DAPI combined PKH26 could be detected in the hepatic tissues. Conclusion BMSC double-labeled by DAPI combined PKH26 in mice present well tracing effect

关 键 词：DAPI PKH26 骨髓干细胞 

分 类 号：R574.5[医药卫生—消化系统]