消黄方对大鼠胆汁淤积性肝纤维化的干预作用  

Therapeutic Effects of XiaoHuangFang on Cholestatic Liver Fibrosis in Rats

作　　者：刘成[1,2] 杨宗国[1,2] 陆云飞[1,2] 陈晓蓉[1,2] 

机构地区：[1]上海市(复旦大学附属)公共卫生临床中心,上海201508 [2]国家中医药管理局中医药防治传染病重点研究室(临床基地)

出　　处：《西部中医药》2012年第11期14-17,共4页Western Journal of Traditional Chinese Medicine

基　　金：国家"十一五"科技重大专项子课题(2009ZX10005-019);上海市科学技术委员会项目(编号25382;10411963000);上海市卫生局项目(编号2011Y020);中央高校基本科研业务费专项资金资助(编号10F115)

摘　　要：目的:观测消黄方对胆管结扎大鼠肝功能及组织病理学的干预作用。方法:Wistar大鼠30只,随机分为假手术组和胆管结扎组(BDL)。BDL大鼠在2周末随机分为消黄方组和模型组,第3周开始消黄方组每天灌胃给药,假手术组和BDL组灌胃等量生理盐水,共3周。5周末处死全部大鼠,获取血清和肝组织样本。结果:与假手术组相比,模型组血清谷草转氨酶(ALT),谷丙转氨酶(AST)和碱性磷酸酶(ALP)活性及总胆红素(TBIL)含量显著上升(P<0.05或0.01),白蛋白(Alb)含量显著下降(P<0.01);与模型组相比,消黄方显著降低ALP活性和TBIL含量,显著抑制Alb含量的降低(P<0.05或0.01),降低肝组织Hyp含量(P<0.05),同时病理学显示消黄方组肝纤维化程度也显著减轻。结论:消黄方能显著抑制胆汁淤积性肝纤维化的形成。Objective： To explore the intervention of XiaoHuangFang on liver function and histopathology of rats with bile duct ligation.Method： Thirty rats were randomized into sham operation group and bile duct ligation（BDL） group.Rats in BDL group were divided into XiaoHuangFang group and model group by the end of two weeks,XiaoHuangFang group began to receive intragastric administration from the third week,sham operation group and BDL group received lavage of same amounts of psychological saline for three weeks.All the rats were sacrificed by the end of fifth week to obtain the serum and liver tissue.Result： Compared with sham operation group,activities of ALT,AST and ALP of model group and contents of TBIL remarkably increased（P0.05 or 0.01）,contents of Alb lowered notably（P0.01）;compared with model group,XiaoHuangFang can lower the activity of ALP and the content of TBIL remarkably,inhibit the decrease of Alb contents（P0.05 or 0.01） and reduce the contents of Hyp in liver tissue（P0.05）,meanwhile pathology of liver fibrosis in XiaoHuangFang group showed that it relieved.Conclusion： XiaoHuangFang can obviously inhibit the formation of cholestatic liver fibrosis.

关 键 词：肝纤维化 消黄方 胆管结扎 

分 类 号：R575.22[医药卫生—消化系统]