中枢组胺能神经介导异氟烷麻醉后认知功能障碍  

Involvement of Central Histaminergic System in Memory Deficits after Isoflurane Anesthesia

作　　者：罗涛[1] 刘志刚[1] 刘敏[2] 赵博[1] 侯家保[1] 夏中元[1] 

机构地区：[1]武汉大学人民医院麻醉科,武汉430060 [2]武汉大学人民医院中心实验室,武汉430060

出　　处：《神经损伤与功能重建》2012年第6期407-410,共4页Neural Injury and Functional Reconstruction

基　　金：国家自然科学基金(No.81271205;81102863);湖北省自然科学基金(No.302131889)

摘　　要：目的:探讨组胺能神经元是否参与异氟烷麻醉引起的认知功能障碍。方法:采用抑制性回避记忆模型研究成年雄性SD大鼠异氟烷麻醉后记忆功能变化,通过下丘脑结节乳头核(TMN)微量注射GABAA受体拮抗剂荷包牡丹碱(BMI)研究组胺能核团参与异氟烷麻醉后记忆受损的神经环路机制,免疫印迹法观察组胺能神经通路对海马BDNF表达的调节作用。结果:异氟烷麻醉导致大鼠抑制性回避测试期的步入潜伏期缩短、海马BNDF表达降低;抑制性回避训练期前给予BMI,可增加麻醉大鼠在测试期的步入潜伏期及海马BNDF的表达。结论:中枢组胺能神经环路以及受该环路支配的BDNF信号通路可能为吸入麻醉后记忆受损的机制之一。Objective： To explore whether histaminergic system participates in memory def- icits after isoflurane anesthesia. Methods： The effect of isoflurane on post-anesthesia memory was investigated in male SD rats with one-trial inhibitory avoidance model. The role of histamin- ergic pathway in isoflurane anesthesia induced memory deficits was explored by microinfusion of GABAA receptor antagnist bicucculline methiodide （BMI） into tuberomammillary nucleus （TMN）. The expression of brain-derived neurotrophic factor （BDNF） in hippocampus was as- sessed by western blot. Results： The step-through latency and the expression of BDNF decreased after isoflurane anesthesia. The changes were reversed by microinfusing of BMI into TMN 10 min before one-trial inhibitory avoidance training. Conclusion： Central histaminergic system and its mediated BDNF expression in hippocampus may participate in the memory deficits after isoflurane anesthesia.

关 键 词：认知功能障碍 异氟烷 组胺能神经 脑源性神经营养因子 

分 类 号：R741[医药卫生—神经病学与精神病学] R614.2[医药卫生—临床医学]