Ⅶ型胶原酶诱导大鼠脑出血模型研究  

Evaluation of Type Ⅶ Collagenase-induced Intracerebral Hemorrhage in Rats

作　　者：倪厚杰[1,2] 刘娜[1] 陈娟[1] 刘永明[1] 张誉[1] 杨二方[1] 唐洲平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030 [2]武汉市第一医院神经内科,武汉430022

出　　处：《神经损伤与功能重建》2012年第6期411-412,共2页Neural Injury and Functional Reconstruction

基　　金：国家自然科学基金(No.81171089);湖北省卫生厅自然科学基金(No.JX4A03)

摘　　要：目的:Ⅶ型胶原酶制作大鼠脑出血模型并评价。方法:Ⅶ型胶原酶立体定向注入大鼠尾壳核,于术后1、3、7d观察大鼠神经行为学、血肿体积及脑组织病理改变。结果:存活大鼠均有神经功能缺损,术后3d最明显;尾壳核有出血灶,术后3d血肿体积最大;血肿及周围脑组织存在典型脑出血后病理改变。结论:Ⅶ型胶原酶制作大鼠脑出血模型是理想的造模方法。Objective. To evaluate the animal model of intracerebral hemorrhage （ICH） induced by injection of collagenase type VII into the right basal ganglia in rats. Methods. Collagenase type Ⅶ was injected into the right basal ganglia of rats by stereotatic technique to induce ICH. The neurological symptoms, volume of hematoma and histopathological changes were evaluated at 1, 3 and 7 d after operation. Results. Neurological deficit was found in all the rats with ICH and the neurological deficit score at 3 d was higher than those at 1 and 7 d after operation （P〈0.05）. The hematoma volume at 3 d was larger than those at 1 and 7 d after operation （P〈 0.05）. Typical histopathological changes of ICH were detected around the hematoma. Conclusion：Collagenase type VII induced ICH in rats is an ideal animal model to mimic the ICH of human.

关 键 词：Ⅶ型胶原酶 脑出血 动物模型 

分 类 号：R741[医药卫生—神经病学与精神病学] R33[医药卫生—临床医学]