地塞米松诱导PC12细胞氧化应激损伤及对NOX2表达的影响  

Effects of dexamethasone on oxidative stress injury and expression of NADPH oxidase 2 in PC12 cells

作　　者：司秀莲[1] 李维祖[1] 李卫平[1,2] 孙立[1] 曹玲玲[3] 熊莉[1] 

机构地区：[1]安徽医科大学药理学教研室,合肥230032 [2]安庆医药高等专科学校,安庆246003 [3]淮南联合大学医学系基础教研室,淮南232038

出　　处：《安徽医科大学学报》2012年第12期1423-1426,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81173624);安徽省科技厅国际合作项目(编号:12030603007);安徽省自然科学基金(编号:11040606M201);安徽医科大学博士启动基金(编号:XJ201011);安徽省教育厅重点项目(编号:KJ2012A192)

摘　　要：目的观察地塞米松(DEX)对大鼠嗜铬瘤细胞(PC12)细胞氧化应激损伤及对烟酰胺腺嘌呤二核苷酸磷酸氧化酶2(NOX2)表达的影响。方法体外培养的PC12细胞随机分为正常对照组、H2O2(100μmol/L)组、DEX(1、5、10μmol/L)组。四甲基偶氮唑盐(MTT)法测细胞存活率,双氢溴化乙啶(DHE)荧光染色法测定细胞内活性氧(ROS)水平。RT-PCR法检测NOX2、小G蛋白(Rac1)mRNA表达。Western blot法检测NOX2、p47phox的表达。结果与正常对照组比较,DEX作用于PC12细胞24 h后,DEX(5、10μmol/L)组细胞活力下降、ROS水平显著增高。进一步研究显示DEX(5、10μmol/L)组可以上调NOX2和Rac1 mRNA的表达,可以增加NOX2、p47phox的表达。结论 DEX可以增加PC12细胞ROS的生成,诱导PC12细胞氧化应激损伤,其机制可能与增加NOX2表达有关。Objective To investigate dexamethasone （DEX） induced PC12 cell death by oxidative stress and its influence on the NOX2 expression. Methods PC12 cells were randomly divided into five groups：control group, H202 （100 p, mol/L） group, DEX（ 1,5,10 p, mol/L）. The survival of PC12 cells was detected by MTI＂ assay. Intra- cellular reactive oxygen species （ROS） was assessed by dihydroethidium （DHE）, NOX2 and small G protein （ Racl ） mRNA level in PC12 cells were detected by RT-PCR. The expression of NOX2, p47phox was examined by Western blot. Results Compared with control group, the survival of PC12 cells in DEX （5, l0 ptmol/L） groups decreased and ROS level increased significantly after 24 h treatment. Furthermore, DEX could upgrade the expres- sions of NOX2 ,Rael mRNA and NOX2, p47phox. Conclusion DEX could increase the generation of intracellular ROS in PC12 cells and induce oxidative stress injury, NOX2. and its mechanism is possibly increased by the expression of NOX2.

关 键 词：氧化应激 NOX2 PC12细胞 地塞米松 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R345.4[医药卫生—临床医学]