脂多糖对人肠微血管内皮细胞水通道蛋白1表达及功能的影响  

Effects of lipopolysaccharide on aquaporin 1 expression and function in human intestinal microvascular endothelial cell

作　　者：陈信浩[1,2] 纪俊标[2] 吕纯业[2] 戴存才 

机构地区：[1]南京医科大学第一附属医院普通外科,江苏省210029 [2]南京医科大学附属江宁医院

出　　处：《江苏医药》2012年第22期2649-2651,共3页Jiangsu Medical Journal

基　　金：南京市科技计划项目(201201085);南京市青卫工程资助项目

摘　　要：目的研究脂多糖(LPS)对人肠微血管内皮细胞(HIMEC)水通道蛋白(AQP)1表达及功能的影响。方法 40份HIMEC均分为A、B、C、D四组。A、B、C组分别经浓度为0.1、1、10mg/L的LPS作用;D组为对照组,只加培养基。各组细胞培养12h。Western blot法检测HIMEC AQP-1的表达;RT-PCR检测HIMEC AQP-1mRNA的表达;放射法测定HIMEC的摄水功能。结果随着作用于HIMEC的LPS浓度增加,AQP-1蛋白及其mRNA表达和HIMEC的摄水功能均逐步减少(P<0.05)。结论 HIMEC AQP-1表达与LPS浓度呈负性相关。Objective To study the effects of lipopolysaccharide（LPS） on aquaporin 1（AQP-1） expression and function in human intestinal microvascular endothelial cells（HIMEC）.MethodsFourty pieces of HIMEC were equally randomized into 4 groups of A（treated with LPS 0.1 mg/L for 12 h）,B（with LPS 1 mg/L）,C（with LPS 10 mg/L） and D（blank control）.The expressions of AQP-1 protein and mRNA in HIMEC were detected by Western blot and RT-PCR,respectively.Water intake of HIMEC was measured by radioactive method.Results As the concentration of LPS increased,the expressions of AQP-1 protein and mRNA in HIMEC were decreased（P0.05）.So did the ability of water intake of HIMEC（P0.05）.Conclusion AQP-1 expression is negatively correlated to LPS concentration.

关 键 词：细菌内毒素 内皮细胞 水通道蛋白1 脂多糖 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]