治疗剂量308nm准分子光对小鼠皮肤的致癌性  

The Carcinogenicity of 308nm Excimer Light Irradiating to the Skin of Mice with Therapeutic Dose

作　　者：周俊[1] 应朝霞[1] 王永贤[1] 马慧群[1] 张淑兰[1] 李蕊联[1] 

机构地区：[1]西安交通大学医学院第二附属医院皮肤科,陕西西安710004

出　　处：《中国皮肤性病学杂志》2012年第12期1055-1059,共5页The Chinese Journal of Dermatovenereology

基　　金：陕西省科学技术研究发展计划项目(2010K01-202);2010年新兴前沿;学科综合交叉项目(xjj20100213)

摘　　要：目的 探究常规治疗剂量308nm准分子光长期照射对小鼠皮肤的致癌性。方法 将96只昆明小鼠根据308nm准分子光照射时间(4周、8周、12周、16周、20周和24周)不同分为6组,每组8只,每个时间组均设正常对照组;参考人非增生性皮肤病变治疗剂量,初始照射剂量60mJ/cm2,1次/周,再根据治疗反应逐渐增加治疗剂量。照射结束后观察小鼠皮肤组织病理变化(HE染色)和免疫组化法检测P53蛋白表达。结果 正常对照组小鼠皮肤组织病理无明显改变,P53阳性表达率随照射时间增加无明显变化(P>0.05);308nm准分子光照射16周后,部分小鼠皮肤开始出现角质形成细胞的非典型增生及鳞状细胞癌病理表现,P53蛋白阳性表达率随照射时间延长而逐渐增加(P<0.05);照射16周、20周和24周时,308nm准分子光组阳性率均高于对照组,差异均有统计学意义(P均<0.05)。结论 308nm准分子光对小鼠皮肤具有一定致癌性,致癌风险同照射时间呈正相关。Objective To explore the carcinogenicity of 308nm excimer light long-term irradiating with therapeutic dose to the skin of mice. Methods A total of 96 Kunming mice were divided into six groups randomly by differ- ent irrndinted time of 308 nm excimer light ,as 4-weeks irradiated group ,8-weeks irradiated group, 12-weeks irradiated group, 16-weeks irradiated group ,20-weeks irradiated group and 24-weeks irradiated group. Nor- ma] control for each group were established. The incipient dose was 60mJ/cm2. Irradiated once a week, and increased the dose gradually depending on the response. After finishing irradiations, the expression of P53 protein was detected by HE staining and immunochemical method. Results In normal control groups, The pathology of cutaneous tissue had no changes, and the positive expression of P53 protein was time-independ- ent（P 〉0.05 ）. But in irradiated groups, atypical hyperplasia and squamous cell carcinoma were observed after 16-weeks excimer light irradiation. The positive expression of P53 protein in irradiated groups was in- creased gradually accompany with irradiation time extending （ P 〈 0.05 ）. There were significant difference a- mong 16-weeks groups, 20-weeks groups and 24-weeks groups（ all P 〈 0.05 ） , and the positive expression rate of P53 protein in excimer light irradiated groups was higher than that in control groups. Conclusion 308nm excimer light irradiating to the skin of mice has carcinogenicity. The longer irradiation time is, the greater the risk of cancer is.

关 键 词：308nm准分子光 小鼠 皮肤癌 P53蛋白 

分 类 号：R73[医药卫生—肿瘤]