肌醇六磷酸对人骨肉瘤U2OS细胞的体外抑制作用  

Anti-tumor effect of phytic acid on human osteosarcoma U2OS cells in vitro

作　　者：王路[1] 程翠林[1] 赵海田[1] 崔洪斌[2] 

机构地区：[1]哈尔滨工业大学食品科学与工程学院,哈尔滨150090 [2]哈尔滨医科大学公共卫生学院

出　　处：《卫生研究》2012年第6期943-946,共4页Journal of Hygiene Research

基　　金：国家自然科学基金(No.30471446);黑龙江省自然科学基金(No.C201037);黑龙江省博士后资助经费(No.LBH-Z10292)

摘　　要：目的探讨肌醇六磷酸体外对人骨肉瘤U2OS细胞的影响及其机制。方法通过检测细胞增殖活性、超微扫描电镜观察、荧光染色法检测细胞凋亡及c-myc蛋白表达来研究肌醇六磷酸对U2OS细胞的影响。结果肌醇六磷酸能够抑制人骨肉瘤细胞U2OS的增殖,扫描电镜观察可见细胞表面超微结构发生凋亡改变,荧光显色证实肌醇六磷酸能够促细胞凋亡,c-myc蛋白的表达降低。结论肌醇六磷酸在体外对人骨肉瘤U2OS细胞增殖的抑制机制可能与诱导细胞凋亡及调控c-myc蛋白表达相关。Objective To explore the effect of phytic acid on the human osteosarcoma U2OS cells and its mechanisms in vitro.Methods MTT assay was used to examine the cell proliferation.Scanning electron microscope（SEM） was used to observe the surface ultrastructure of U2OS cells.Hoechst 33342 fluorescence staining assay were applied to study the pro-apoptosis effects.The immune histochemisty method was used to detect c-myc expression.Results Phytic acid could significantly inhibit the growth of U2OS.The surface ultrastructure of U2OS cells showed distinct morphological changes corresponding to a typical cellular surface morphology of apoptosis.From fluorescence staining,we observed chromoplasm pyknosis and apoptotic body.Expression of c-myc protein decreased.Conclusion The anti-osteosarcoma mechanism of phytic acid may be related to its influence on inducing apoptosis and adjusting c-myc expression.

关 键 词：肌醇六磷酸 C-MYC 人骨肉瘤U2OS细胞 

分 类 号：R730.1[医药卫生—肿瘤] R730.269[医药卫生—临床医学]