环磷酰胺和氢化可的松建立免疫抑制模型方法比较  

Comparison of experimental murine immunodeficiency models induced by cyclophosphamide and hydrocortisone

作　　者：吕颖坚[1] 黄俊明[1] 蔡玟[1] 黄琼[1] 黄建康[1] 李文立[1] 陈壁锋[1] 胡帅尔[1] 

机构地区：[1]广东省疾病预防控制中心卫生毒理所,广州510300

出　　处：《卫生研究》2012年第6期951-955,共5页Journal of Hygiene Research

基　　金：保健食品功能评价方法及有关程序修订项目(2009)

摘　　要：目的比较利用环磷酰胺(CP)和氢化可的松(HY)两种药物诱导建立小鼠免疫抑制模型的方法。方法设立CP-1组、CP-2组、CP-3组和HY组,相对应地设4个阴性对照组。CP-1组于第1、2、3、9、16和23天腹腔注射80mg/kg CP;CP-2组第1、3、5、7和9天腹腔注射50mg/kg CP;CP-3组d1、d2腹腔注射40mg/kg CP;HY组d1、d3、d5、d7和d9肌内注射40mg/kg HY。阴性对照-1、2、3组腹腔注射等剂量生理盐水,阴性对照-4组肌内注射等剂量生理盐水。分别于第29、11、7和10天测定各项免疫指标。结果CP组小鼠胸腺指数、抗体生成细胞数、半数溶血值(HC50)、白细胞计数与阴性对照组比较均降低(P<0.05)。HY组小鼠脾指数、胸腺指数、碳廓清吞噬指数、腹腔巨噬细胞吞噬荧光微球功能、NK细胞活性、迟发型变态反应、白细胞计数与阴性对照组比较均降低(P<0.05)。结论 CP和HY均可建立小鼠免疫抑制模型,各种指标对两种受试物的敏感性不同。Objective To compare the experimental murine immunodeficiency models induced by cyclophosphamide（CP） and hydrocortisone（HY）,and choose suitable animal models for function evaluation of health food.Methods 7 trials were performed：evaluation of spleen and thymus index and WBC count,evaluation of the phagocytic function of the reticuloendothelial system,evaluation of macrophage phagocytosis function,splenic natural killer（NK） activity,delayed type of hypersensitivity response,plaque-forming cell assay and assay of serum hemolysin to SRBC.The animals were randomly divided into 2 groups of 12 mice for each experimental series.Group 1 served as vehicle control and Group 2 served as the CP/HY treated control.CP-1 at 80mg/kg was administered intraperitoneally on day1,2,3,9,16,and 23.CP-2 at 50mg/kg was administered intraperitoneally on day1,3,5,7,and 9.CP-3 at 40mg/kg was administered intraperitoneally on day1 and 2.HY injection at 40mg/kg was administered intramuscularly on day1,3,5,7,and 9.All groups were used for different immunomodulatory activity screening experiments,respect on day 29,11,7,and 10.Results Compared to vehicle control,the plaque-forming cells,serum hemolysin and WBC count were reduced in all CP groups.While the phagocytic function of the reticuloendothelial system,macrophage phagocytosis function,NK activity,delayed type of hypersensitivity response and WBC count were reduced in HY group.Conclusion Both CP or HY could build a murine immunodeficiency model and they have different sensitivity indexes.

关 键 词：环磷酰胺 氢化可的松 小鼠免疫抑制模型 

分 类 号：R155.5[医药卫生—营养与食品卫生学] TS218[医药卫生—公共卫生与预防医学]