小菜蛾高致病力玫烟色棒束孢菌株的筛选  

Screening of High Virulent Strain of Isaria fumosorosea Against Diamondback Moth,Plutella xylostella

作　　者：刘玉军[1] 陈名君[1] 汤强[1,2] 王滨[1] 黄勃[1] 

机构地区：[1]安徽农业大学微生物防治省重点实验室,安徽合肥230036 [2]芜湖职业技术学院,安徽芜湖241003

出　　处：《热带作物学报》2012年第10期1856-1860,共5页Chinese Journal of Tropical Crops

基　　金：农业公益性行业科研专项(No.201303029);林业公益性行业科研专项(No.201204506);环保行业科研专项(No.201009023);安徽省高校自然科学基金项目(No.KJ2012B218)

摘　　要：小菜蛾(Plutella xylostella)是全球十字花科蔬菜上的重要害虫,并且对常用的化学杀虫剂产生了严重的抗性,每年给农业蔬菜带来了巨大损失。本研究选择分离自不同鳞翅目的 8个玫烟色棒束孢(Isaria fumosorosea)菌株,依据菌株生长速度、产孢量、萌发率和产胞外蛋白酶水平等生物学性状进行初步筛选。在此基础上,筛选出生物学性状优良菌株RCEF2180、RCEF3423、RCEF3496对小菜蛾幼虫进行生物测定。最后筛选出了生长速度快、产孢能力强、萌发率高、胞外蛋白酶产酶水平高及杀虫效果好的玫烟色棒束孢RCEF3423菌株。该菌株对小菜蛾的校正死亡率达96.16%,感染率达91.67%,在浓度为1×107孢子/mL时的半致死中时仅为4.58 d,显示出对小菜蛾的极强毒力,因此该菌株在小菜蛾的生物防治中具潜在的应用价值。The diamondback moth （DBM）, Plutella xylostella （L.）（Lepidoptera： Plutellidae）, is one of the most destructive cosmopolitan insect pests of brassicaceous crops. It is the first crop insect reported to be resistant to DDT and now, in many crucifer producing regions, it shows significant resistance to almost every synthetic insecticide applied in the field. The present study examined the difference of growth rate, sporulation, germination rate, and extracellular protease production among eight Isaria fumosorosea strains isolated from Lepidoptera in China. Three strains RCEF2180, RCEF3423 and RCEF3496 with good biological characters were selected for further bioassay against the larvae of P. xylostellais. The strain RCEF3423 showed high virulence to P. xylosteUais with a corrected mortality of 96.16%, a infection ratio of 91.67%, and a lethal time for 50% mortality （LT50）of 4.58 d at a concentration of 1 ×10^7 conidia/mL. The strain showed great potential for sustainable for use in microbial control of P. xrlostella.

关 键 词：小菜蛾 玫烟色棒束孢 生物学特性 生物测定 菌株筛选 

分 类 号：S436.341.2[农业科学—农业昆虫与害虫防治]