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机构地区:[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃省草业工程实验室,甘肃兰州730070
出 处:《植物营养与肥料学报》2012年第6期1537-1544,共8页Journal of Plant Nutrition and Fertilizers
基 金:国家自然科学基金项目(30671267,31060261);甘肃省科技厅自然科学基金项目(1010RJZA164)资助
摘 要:以裸仁美洲南瓜抗、感病品种为材料,测定了硅酸钠溶液喷雾处理南瓜幼苗后诱导接种白粉菌对不同抗病品种裸仁美洲南瓜叶片中酚类物质、绿原酸、类黄酮3种物质在不同处理时期含量变化的影响。结果表明,硅酸钠诱导处理后不同抗性品种南瓜叶片中的酚类物质、绿原酸、类黄酮等物质含量均明显升高,抗病品种中酚类物质含量在第5 d、7 d、9 d时分别高于对照和接菌处理的,绿原酸含量在各个处理时间均高于对照和接菌处理的,类黄酮含量在第7 d、9 d时分别高于对照和接菌处理的;感病品种中酚类物质含量和绿原酸含量分别在第5 d、7d和9 d时高于对照和接菌处理;类黄酮含量在第3 d、5 d、7 d和9 d时均高于其他处理;硅酸钠诱导处理后,大多数情况下抗病品种的酚类物质(除第7 d外)、绿原酸(除第7 d、9 d外)、类黄酮等含量均高于感病品种,差异显著,且在接种后第10 d和第15 d白粉病病情指数明显下降。The contents of phenolic, chlorogenic acids and flavonoid in leaves of resistance and susceptible pumpkin cultivars were determined after those cuhivars were treated by sodium silicate solution foliar spray and the inoculation of Podosphaera xanthii. The results indicate that the contents of phenolic, chlorogenic acids and flavonoid in the leaves of Si supplied plants are significantly increased. The contents of phenolic acid in leaves of the resistant pumpkin cuhivars of the induction treatments( Si + Px) are higher than those of the control (CK) and the inoculation treatments (Px) in 5 d,7 d and 9 d respectively, the contents of chlorogenic acid in leaves of the resistant pumpkin cultivars of the induction treatments ( Si + Px) are higher than those of the CK and the inoculation treatments (Px) in 1 d, 3 d, 5 d, 7 d and 9 d respectively, and the contents of flavonoid in leaves of the resistant pumpkin cultivars of the induction treatments ( Si + Px) are significantly higher than those of the CK and the inoculation treatments (Px) in 7 d and 9 d. The contents of phenolic and chlorogenic acids in leaves of susceptible pumpkin cuhivars of the induction treatments (Si + Px) are higher than those of the CK and the inoculation treatments (Px) in 5 d, 7 d and 9 d respectively, and the contents of flavonoid in leaves of the susceptible pumpkin cultivars of the induction treatments (Si + Px ) are significantlv hither than those of the CK and theinoculation treatments (Px) except in ld. The contents of phenolic (except 7 d), chlorogenic acids (except 7 d and 9 d ) and flavonoid in leaves of the resistant pumpkin cultivars are significantly higher than those of the susceptible cuhivars after treated with sodium silicate induction, the powdery mildew disease indexes of resistance and susceptible pumpkin are decreased significantly at 10 d and 15 d after the inoculation.
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