重组植物甜蛋白马宾灵的食品级乳酸乳球菌诱导表达系统的构建  

Construction of Food-grade Inducible Expression System for Recombinant Plant Sweet Protein Mabinlin Ⅱ in Lactococcus lactis

作　　者：顾文亮[1] 夏启玉[2] 姚晶[1] 胡新文[1] 符少萍[2] 郭建春[2] 

机构地区：[1]海南大学农学院,海口570228 [2]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《中国农学通报》2012年第30期196-200,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"马宾灵基因(Mabinlin)体外甜味表达及其结构发生规律研究"(31060123)

摘　　要：为了将马宾灵(Mabinlin Ⅱ)直接应用于功能性食品中,通过对Mabinlin Ⅱ基因进行有目的的剪切重组,将重组Mabinlin Ⅱ基因插入到食品级表达载体中,经电击转化和乳糖筛选获得重组的食品级乳酸乳球菌,构建了重组植物甜蛋白马宾灵(MBL-ABH)的食品级乳酸乳球菌诱导表达系统。结果表明,该食品级诱导表达系统经乳酸链球菌素(Nisin)诱导后所表达的目的蛋白的含量较低,Western-blot检测表明MBL-ABH成功的在食品级乳酸乳球菌中表达。本研究扩展了植物甜蛋白马宾灵在食品方面的应用,有望开发出能够避免添加食糖类物质的功能性食品。In this study, in reconstructed and then the After electroporation and order to directly use Mabinlin Ⅱ recombinant Mabinlin Ⅱ gene was lactose utilization screening, the in functional food, the Mabinlin Ⅱ gene was inserted into the food-grade expression vector. food-grade inducible expression system for recombinant plant sweet protein Mabinlin Ⅱ in L. lactis was constructed. The expression of the induced recombinant Mabinlin Ⅱ was in a low level. The western blotting results demonstrated that the recombinant Mabinlin Ⅱ protein was successfully expressed by the recombinant food-grade L. Iactis when the inducible expression system was induced by Nisin. This research showed the feasibility of constructing a food-grade L. lactis dairy product strategy by removing the need for addition of sugar and contributing to the value of functional food.

关 键 词：植物甜蛋白 马槟榔 马宾灵 乳酸乳球菌 蛋白表达 

分 类 号：Q812[生物学—生物工程]