拮抗细菌GF-312的复合诱变选育  

Composite Mutation Breeding of Antagonistic Bacteria GF-312

作　　者：高菲[1] 卜春亚[1] 靳永胜[1] 王有年[1] 师光禄[1] 

机构地区：[1]北京农学院/农业部都市农业(北方)重点实验室,北京102206

出　　处：《中国农学通报》2012年第30期254-258,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金资助项目(30872029);北京市自然科学基金重点项目(5111001);北京市自然科学基金项目(6092007);北京市教委重点项目(KZ201010020016);北京市教委平台建设项目(PXM2010_014207_098661);北京市属高校人才强教深化计划资助项目(PHR20090516);北京市属高校人才强教深化计划资助项目(PHR200906134)

摘　　要：为了提高已筛选出的拮抗菌株的抑菌活性,以荧光假单胞菌(Pseadomonas fluorescens)GF-312为出发菌株,采用紫外线和微波方法对其进行复合诱变,通过对致死率、正突变率和抑菌活性的测定,确定紫外线和微波诱变的最适时间,结合对诱变菌株的传代稳定性测定,获得高效菌株GF-16,其发酵液的抑菌圈直径达到30.4mm,抗菌活性比紫外诱变之前(自然选育之后)的菌株GF-51提高了18.8%。In order to improve the antibacterial activity of antagonistic bacteria has been screened, as a initialstrain, Pseadomonas fluoreseens GF-312 was mutated by UV and MW. Based on the lethal rate, the positive mutation rate and the antagonistic activity, the best optional time of UV and microwave irradiation were determined. Combining with the genetic stability of strains selected, the high efficient strain named GF-16 was obtained. The result of antifungal aetivity showed that the diameter of inhibition reaehed up to 30.4 ram, and the activity increased by 18.8%.

关 键 词：荧光假单胞菌GF-312 诱变 紫外线 微波 抑菌活性 

分 类 号：S436.639[农业科学—农业昆虫与害虫防治]