牙鲆变态发育过程中epigen基因的空间表达分布  

Expressional pattern of epigen gene in metamorphosing Paralichthys olivaceus using whole-mount in situ hybridization

作　　者：李乐康[1] 鲍宝龙[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《上海海洋大学学报》2012年第6期929-934,共6页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(31072201);上海市教育委员会重点学科建设项目(S30701)

摘　　要：细胞分裂在鲽形目鱼类变态过程中发挥了重要作用。Epigen作为表皮分裂原,通过激活MAPK通道来调控有丝分裂。以前的研究利用定量PCR表明,epigen在变态前期表达量升高,而变态后期表达量下降,表明其与变态发育相关。为了进一步调查epigen与牙鲆变态发育的关系,利用RNA整体原位杂交技术检测了epigen在变态期牙鲆体内的空间表达分布,发现在变态期的各个阶段,epigen基因表达分布均比较广泛,表皮、鳃、肝脏、肠道、鳍条、支鳍骨、肌节等组织都有表达,在牙鲆变态前和变态早期(E期)信号较强,而在变态高峰期(F期和G期)和变态后期(H期)信号逐渐变弱。Epigen空间表达分布模式提示,其作为表皮分裂原,可能广泛参与牙鲆变态发育早期事件,尤其可能与肝脏、肠道、支鳍骨和鳍条的发育有关。Cell proliferation plays important roles during metamorphosis in flatfishes.As an epithelial mitogen,epigen can promote cell proliferation in a dose-dependent manner via stimulating phosphorylation of the EGFR and downstream signaling molecules such as the mitogen activated protein kinase（MAPK）.Q-RT PCR result in a previous study showed that epigen expressed higher in early stage of metamorphosis,and became low in climate stage of metamorphosis.To further investigate the relationship between epigen expression and metamorphic events in Paralichthys olivaceus,we employed whole-mount in situ hybridization to detect the expressional pattern of epigen gene during metamorphosis.The results indicated that epigen expressed universally in various tissues.The expressional signal of epigen in larvae during pre-metamorphosis to Stage E was strong,then became weak after Stage F.It was observed that epigen expressed mainly in gill,liver,intestines,fin,actinost,myotome and epidermis.Tempo-spatial expressional patten of epigen gene in P.olivaceus indicates that it might participate in some metamorphic events,particularly in those events that happened during early stages of metamorphosis.

关 键 词：牙鲆 变态 EPIGEN 整体原位杂交 

分 类 号：S917[农业科学—水产科学]