结直肠癌中Tiam1基因启动子区甲基化与其表达的相关性  

The correlation between aberrant methylation of Tiam1 promoter and its expression in colorectal cancer

作　　者：王博[1] 李婷婷[1] 金贺[1] 林洁[1] 丁彦青[1] 

机构地区：[1]南方医科大学基础医学院病理学系,广州510515

出　　处：《广东医学》2012年第22期3355-3359,共5页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30800414)

摘　　要：目的分析结直肠癌中Tiam1基因启动子区甲基化情况与其蛋白表达之间的关系。方法采用亚硫酸氢盐修饰测序法(bisulfite sequencing PCR)检测2例结直肠癌细胞系、4例结直肠癌组织及其配对的正常结直肠黏膜中Tiam1基因启动子区CpG岛的甲基化情况;采用免疫组织化学S-P法检测Tiam1蛋白的表达。结果Tiam1基因启动子的CpG位点在4例结直肠癌组织、2株结直肠癌细胞中均未甲基化,而在正常结直肠黏膜中发生了甲基化;Tiam1蛋白在结直肠癌组织及细胞中表达,在正常肠黏膜组织中无表达。结论结直肠癌中Tiam1基因启动子低甲基化与其蛋白高表达间呈负相关性,Tiam1基因启动子的异常甲基化很可能在Tiam1促进结直肠癌侵袭与转移中发挥重要作用。Objective To investigate the correlation between aberrant methylation of Tiam1 promoter and its ex- pression in colorectal cancer （CRC）. Methods Bisulfite sequencing PCR was applied to assess the methylation status of Tiam1 promoter in two CRC cell lines （ SW620, SW480）, CRC tissues and paired normal mucosa from four cases of CRC. Immunohistochemical （S -P） method was carried on to detect the expression of Tiam1 in the above tissues and cell lines. Results No CpG methylation of Tiam1 promoter was detected in all four CRC samples and two CRC cell lines, while hypermethylation in paired normal mucosa was observed. Meanwhile, Tiam1 protein was overexpressed in CRC tissue and CRC cell lines, while it was negative in paired normal mucosa. Conclusion Hypomethylation of Tiam1 promoter is related to over expression of Tiam1 protein in CRC, suggesting that aberrant methylation of the Tiam1 promoter plays an important role in CRC development.

关 键 词：亚硫酸氢盐修饰测序法 CPG岛 甲基化 Tiam1蛋白 

分 类 号：R735.2[医药卫生—肿瘤]