喂饲转HJC-1和G6-EPSPS基因大米大鼠主要营养素吸收利用率试验研究  

Main nutrients absorption availability of transgenic rice of HJC-1 and G6-EPSPS in rats

作　　者：刘洪亮[1,2] 王洋[1] 张明月[3] 王静[3] 姜文玲[3] 

机构地区：[1]天津市疾病预防控制中心环境与健康所,天津300011 [2]天津医科大学公共卫生学院 ,天津300011 [3]天津市疾病预防控制中心卫生检验所 ,天津300011

出　　处：《环境与健康杂志》2012年第11期963-966,共4页Journal of Environment and Health

基　　金：国家“十一五”转基因重大专项(2008ZX08011-005)

摘　　要：目的研究转HJC-1和G6-EPSPS基因大米大鼠主要营养素吸收利用率。方法将140只Wistar大鼠随机分为7组,每组20只,雌雄各半,即转基因大米低、中、高剂量3个组,亲本对照低、中、高剂量3个组和饲养对照组,相应饲料喂养7 d。比较各组大鼠蛋白质功效比值及蛋白质、脂肪、碳水化合物、纤维素的表观消化率等。结果除雄性亲本高剂量组蛋白质功效比值高于饲养对照组外,其他各组蛋白质功效比值与饲养对照组差别无统计学意义;除雌鼠转基因低剂量组的蛋白质表观消化率和雄鼠亲本低剂量组的脂肪表观消化率外,其他各组的蛋白质表观消化率、脂肪表观消化率、碳水化合物表观消化率、纤维素表观消化率均高于饲养对照组(P<0.01);转基因各组与相应亲本对照组上述指标的差异均无统计学意义。结论喂饲转HJC-1和G6-EPSPS基因大米和亲本对照大米的大鼠主要营养素(蛋白质、脂肪、碳水化合物、纤维素)吸收利用率基本具有实质等同性。Objective To study the main nutrients absorption availability of transgenic rice of HJC-I and G6-EPSPS in rats. Methods Wistar rats were randomly divided into seven groups, three groups of transgenie rice, three groups of non- transgenic rice and control group. The rats were fed with corresponding food for seven days. Apparent digestibility of protein, fat, carbohydrate,fiber, and protein efficiency ratio were compared. Results There were no significant difference in protein efficiency ratio between all groups except non-transgenic high group and control group（P〉0.05 ）. Protein apparent digestibility, fat apparent digestibility, carbohydrate apparent digestibility and fiber apparent digestibility in all groups were higher compared with those of the control group, except protein apparent digestibility in low group and fat apparent digestibility in non-transgenie low group （P〈0.01）. Conclusion There is substantial equivalence in absorption availability of the main nutrients （protein, fat, carbohydrate, fiber） between transgenic rice and non- transgenie rice in rats.

关 键 词：转基因大米 HJC-1基因 G6-EPSPS基因 营养素 吸收利用率 大鼠 

分 类 号：R994.6[医药卫生—毒理学]