柑桔裂皮类病毒江西分离物(CEVd-RJ)全长cDNA序列分析  

Sequence Analysis of the Full Length cDNA of a Citrus exocortis viroid CEVd-RJ Jiangxi Isolate

作　　者：易龙[1,2] 陶珍珍 卢占军 钟八莲[2] 

机构地区：[1]赣南师范学院生命与环境科学学院,赣州341000 [2]江西省脐橙工程技术研究中心

出　　处：《中国南方果树》2012年第6期1-4,共4页South China Fruits

基　　金：江西省青年科学家培养计划(2010DQ02300);江西省科技支撑计划项目(2010BNB01402)资助

摘　　要：运用RT-PCR技术对柑桔裂皮类病毒江西分离物CEVd-RJ的全长cDNA序列进行扩增、克隆,并对其片段进行序列测定。将获得的371bp的全长cDNA序列与GenBank登录的11个柑桔裂皮类病毒相应序列进行比对分析,结果发现CEVd-RJ与湖北分离物CEVd-HB的同源性为99.7%,与广东分离物CEVd-YC的同源性为90.8%,同国外9个分离物间的同源性在89.0%～98.6%之间。序列差异分析发现,CEVd-RJ的全长cDNA序列比CEVd-YC少一个碱基,有36个位点碱基发生了变化,与CEVd-HB只存在一个碱基位点的差异。The Citrus exocortis viroid,CEVd-RJ,isolated from Jiangxi province was amplified using RT-PCR,and the full length cDNA was cloned and sequenced.Comparison with the 11 citrus exocortis viroid isolates deposited in GenBank revealed that the Jiangxi isolate,CEVd-RJ,shared 99.7% of identity with the Hubei isolate,CEVd-HB,and 90.8% of identity with the Guangdong isolate,CEVd-YC,in their cDNA sequences.CEVd-RJ also shared 89.0% to 98.6% cDNA identities with 9 isolates from other countries.Compared with CEVd-YC,CEVd-RJ had a 1 bp deletion and 36 nucleotide changes in its full length cDNA.Notably,there was only one nucleotide difference between CEVd-RJ and CEVd-HB.

关 键 词：柑桔裂皮类病毒 江西 全长CDNA 序列分析 

分 类 号：S432.41[农业科学—植物病理学]