玉叶金花水提物的抗炎抑菌作用  

Studies on Anti-inflammatory and Antibacterial Activities of Aqueous Extract from Mussaenda pubescens

作　　者：潘利明[1,2] 林励[2] 胡旭光[1] 

机构地区：[1]广东药学院,广州510006 [2]广州中医药大学,广州510006

出　　处：《中国实验方剂学杂志》2012年第23期248-251,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究玉叶金花水提物的抗炎、抑菌作用。方法:将小鼠随机分为对照组、阳性对照组和玉叶金花水提物高、中、低剂量组(33.6,16.8,8.4 g·kg-1),ig 4 d,以二甲苯致小鼠耳肿胀模型观察其抗炎作用;将大鼠随机分为对照组、阳性对照组和玉叶金花水提物高、中、低剂量组(24,12,6 g·kg-1),以角叉菜胶致大鼠足趾肿胀模型及大鼠棉球肉芽肿模型观察其抗炎作用;通过体外抑菌试验,观察玉叶金花提取物的抑菌作用。结果:玉叶金花水提物的中、高剂量能显著减轻二甲苯致炎小鼠的耳肿胀度(P<0.05);玉叶金花水提物中、高剂量可明显抑制由角叉菜胶引起的大鼠2,3 h的足肿胀度(P<0.05);玉叶金花水提物给药组肉芽肿的质量较空白对照组明显减少;玉叶金花水提物对金黄色葡萄球菌、大肠埃希菌、肺炎球菌、链球菌、痢疾杆菌均有抑菌作用,其最小抑菌浓度(MIC)分别为125,31.3,15.7,62.5,62.5 mg·kg-1。结论:玉叶金花水提物具有明显的抗炎及抗菌作用。Objective： To study the antiphlogistic and antibacterial effect of aqueous extract from Mussaenda pubescens. Method： Mice were divided into 5 groups randomly ： normal saline （NS） group, positive group, high dose group, middle dose group and low dose group of aqueous extract from M. pubescens （33.6, 16.8, 8.4 g .kg^-1 ）. After 4 d by ig administration, dimethybenzene-induced mouse ear edema model was used to evaluate the anti-inflammation effect. Rats were divided into 5 groups randomly： normal saline （NS） group, positive group, high dose group, middle dose group and low dose group of aqueous extract from M. pttbescens （24, 12, 6 g·kg^-1）. Using models of carrageenan-induced paw edema and cotton-pellet-induced granuloma, anti-inflammation effect was evaluated. Bacteriostasis effect of aqueous extract from M. pubescens was investigated by the method of bacteriostatic test in vitro. Result： The middle and high dose of the extract could significantly relieve ear-swelling caused by dimethylbenzene （P 〈 O. 05 ）. The middle and high dose of the extract could significantly inhibit paw swelling in rats within 2, 3 hours （P 〈 0.05 ）. The granuloma quality was significantly reduced by the extract compared with the control group （P 〈 0.05 ）. The aqueous extract had antibacterial effects on Staphylococcus aureus, Escherichia coli, Pneumococcus, Streptococcus, Shigella, and the minimum inhibitory concentration （MIC） was 125, 31.3, 15.7, 62.5, 62.5 mg ·kg^-1, respectively. Conclusion： Aqueous extract from M. pubescens has significant anti-inflammatory and antibacterial effects.

关 键 词：玉叶金花 抗炎 抑菌 耳肿胀 足肿胀 肉芽肿 

分 类 号：R285.5[医药卫生—中药学]