基因扫描分析非小细胞肺癌病人外周血和骨髓T细胞克隆性增殖及分布特点  

The feature of clonal expansion and distribution of TCR VβT cells from peripheral blood and bone marrow in patients with NSCLC

作　　者：吴一龙[1] 杨学宁[1] 李锦添[1] 王思愚[1] 

机构地区：[1]中山医科大学肺癌研究中心,广东广州510060

出　　处：《癌症》2000年第3期204-207,共4页Chinese Journal of Cancer

基　　金：卫生部科学研究基金!(98-2-377)资助

摘　　要：目的 :分析非小细胞肺癌 (non smallcelllungcancer ,NSCLC)病人的T细胞受体 (Tcellreceptor ,TCR)Vβ亚家族T细胞的分布及其克隆性增殖特点。方法 :利用RT PCR和基因扫描方法分析 3例NSCLC外周血和骨髓单个核细胞中 2 4个TCRVβ基因的CDR3长度 ,了解TCRVβT细胞的分布及其克隆性。 结果 :3例病人仅存在 1～ 5个TCRVβ亚家族 ,主要为Vβ3、Vβ7和Vβ9,2例病人出现Vβ7寡克隆性增殖T细胞 ,另 1例发现Vβ3的寡克隆T细胞。结论 :NSCLC病人外周血或骨髓的TCRVβ亚家族出现倾斜性分布和克隆性增殖T细胞 ,主要为Vβ3和Vβ7亚家族T细胞 。Objective:To investigate the distribution and clonal expansion of TCR Vβsubfamily T cells in patients with non small cell lung cancer (NSCLC)Methods:The CDR3 size of TCR Vβ24 subfamily genes were analyzed in peripheral blood and bone marrow mononuclear cells from 3 cases with NSCLCusing RTPCR and genescan, to evaluate the distribution and clonality of TCR VβT cellsResults:Only 1～5 Vβsubfamily T cells could be identified in NSCLC cases, the Vβsubfamily T cells main expressed were Vβ3, Vβ7 and Vβ9Vβ7 oligoclonal T cells were identified in two cases, whereas Vβ3 oligoclonal T cells were detected in the other caseConclusions:The skew distribution and clonal expansion of TCR Vβsubfamily T cells could be found on patients with NSCLC, predominantly in Vβ3 and Vβ7It may be the T cell clones for specific immune response due to the lung cancer cellsassociatedantigen

关 键 词：T细胞受体 VΒ基因 T细胞 克隆 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]