高碘对多代仔鼠脑发育和智力影响的实验研究  

The experimental study of high iodine on brain development and intelligence in different generations of mice

作　　者：李英华[1] 朱惠民[1] 郭影[2] 董少霞[2] 刘福英 刘勖[4] 张建军[4] 

机构地区：[1]河北医科大学环境卫生学教研室,石家庄050017 [2]河北医科大学附属三院 [3]河北医科大学实验动物学部 [4]河北医科大学卫生统计教研室

出　　处：《中国地方病防治》2000年第2期68-70,共3页Chinese Journal of Control of Endemic Diseases

摘　　要：目的通过对高碘组仔鼠脑组织结构和智力测试的观察，探讨高碘对脑发育和智力的影响。 方法将小鼠随机分为适碘组和高碘组，分别饮含碘５０μｇ／Ｌ和３０００μｇ／Ｌ的去离子水。连续传三代，观察高碘对各代仔鼠脑发育和智力的影响。结果１．高碘组１５日龄仔鼠大脑和海马绝对重量和相对重量均较适碘组低，但尚无统计学意义（Ｐ＞０．０５）。２．图象分析：高碘组仔鼠大脑皮质锥体层锥体细胞平均体积、平均表面积均较适碘组显著降低（Ｐ＜０．０５），而锥体细胞数密度较适碘组增加（Ｐ＜０．０５）。提示：锥体细胞发育落后。高碘子三代与子一代相比，锥体细胞平均体积进一步减小（Ｐ＜０．０５），数密度进一步增加（Ｐ＜０．０５），提示随着传代次数增多，锥体细胞发育落后程度加重。３．水迷宫实验证实，子一代至于三代，高碘组仔鼠穿越迷宫时间延长，错误次数增加。结论高碘对小鼠脑发育和智力有一定损伤作用，这种损伤随传代次数增加而加重。Objectrve To Study the effect of high iodine on brain development and intelligence, the mare ban Structure and intelligence test were observed. Method The mice were divided into two groups by randomized design. One group drank 50μg/L and the other drank 3000μg/L iodine ion water. Results 1 .The absolute and relatrve weight of brain and hip- pocampus for 15 - day mice and 30- day the in high iodine groups was lower than that in imddle group,but no significant differences were found (P > 0.05). 2.Stereological analysis showed: The mean volume() and mean surface () of cortex pyramidal cells in high iodine were much lower than those in middle iodine groups(P < 0.05). But the numerical density (Nv) was higher than that in middle iodine group(P < 0.05). These findings showed that the development of pyramidal cells was later in high iodine. It was interesting that the pyramidal cells mean volume of the third generation was smaller and the mean numerical density wsa higher than that of the first generation in high iodine groups(P < 0.05). It suggested that high iodine had caused more damage with the increase of generations. 3.The results of water- maze demonstrated :From the first generation to the third generation in high iodine group ,the mice spent longer time and made more errors. Conclusion High io- dine bad done barm to mice brain development and the and the damage was more serious with the increase of genera- tions.

关 键 词：高磺 脑发育 智力 损伤 实验研究 

分 类 号：R151[医药卫生—营养与食品卫生学]