一种紧张型头痛动物模型的建立与评价  

ESTABLISHING AND EVALUATING THE ANIMAL MODEL OF TENSION-TYPE HEADACHE

作　　者：胡志强[1] 吴宏赟[1] 全伟[2] 

机构地区：[1]山东中医药大学附属医院神经内科,济南250011 [2]临沂市中医医院,临沂276002

出　　处：《中国疼痛医学杂志》2012年第11期678-681,共4页Chinese Journal of Pain Medicine

基　　金：山东省自然科学基金项目(编号:Y2008C182)

摘　　要：目的:评价大鼠双侧半棘肌注射三磷酸腺苷(adenosine triphosphate,ATP)建立紧张型头痛动物模型的可行性。方法 :将37只大鼠分为3组,麻醉大鼠暴露双侧半棘肌后,假手术组(n=8)给予针头刺入不注射药物;对照组(n=16)注射生理盐水(20μl/min,持续1 min),ATP组(n=13)注射ATP(20μl/min,持续1 min)。给予舌肌电刺激并在二腹肌记录张颌反射(jaw-opening reflex,JOR),测定JOR出现的阈值、潜伏期。结果 :与注射前相比,注射后假手术组和对照组疼痛阈值无差异(P>0.05),注射后ATP组疼痛阈值减低(P<0.05)。注射后ATP组疼痛阈值低于假手术组和对照组疼痛阈值(P<0.05)。3组注射前后疼痛潜伏期均未出现显著性变化(P>0.05)。结论 :ATP模型模拟紧张型头痛颈部肌筋膜伤害性传入增强发病机制,科学可靠,简单易于复制。Objective： To evaluate the feasibility of establishing an animal model of tension-type headache（TTH） with injecting ATP into semi-spinal neck muscles.Methods： After exposing semi-spinal neck muscles of anesthetized rats,the rats were divided into three groups.In sham group,needles were inserted into the muscle but no injection.In control group,normal saline（20 μl/min,lasting 1 min） was injected into semi-spinal neck muscles.In ATP group,ATP（20 μl/min,lasting 1 min） was injected into semi-spinal neck muscles.The myofascial sensitivity changes were measured by stimulating muscles of tongue,and the pain threshold and latency of jaw-opening reflex（JOR） were recorded in the digastric muscles.Results： There were no obvious difference in pain thresholds before and after injection in the sham group and control group（P 0.05）.Compared with pre-injection,pain thresholds were obviously reduced after injection in the ATP group（P 0.05）.Pain thresholds in ATP group were lower than that in sham and control groups（P 0.05）.There was no significant difference in latency before and after injection in all groups（P 0.05）.Conclusion： The ATP model imitates the pathogenesis of TTH that neck myofascial nociceptive inputs increase,and is scientific,reliable,convenient and easy to be modeled.

关 键 词：紧张型头痛 动物模型 半棘肌 张颌反射 疼痛阈值 

分 类 号：R741[医药卫生—神经病学与精神病学]