豹皮樟总黄酮调控瘦素、TGF-β1对肝纤维化大鼠的预防作用研究  

Regulatory effects of Litsea Coreana level on the expressions of Leptin and TGF-β1 to prevent the hepatic fibrosis in rats

作　　者：黄成[1] 马陶陶[1] 李浩[1] 李政通[1] 章胜朋[1] 邓子煜[2] 李俊[1] 

机构地区：[1]安徽医科大学药学院,安徽天然药物活性研究省级重点实验室,国家中医药管理局中医药三级实验室“中药药理实验室”,安徽合肥230032 [2]安徽医科大学第二附属医院,安徽合肥230601

出　　处：《中国药理学通报》2012年第12期1666-1671,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81072686);安徽省高等学校省级自然科学研究项目(No KJ2010A162);安徽省自然科学基金重点项目(No KJ2012A156)

摘　　要：目的研究豹皮樟总黄酮(TFLC)调控瘦素(leptin)和转化生长因子-β1(TGF-β1)对肝纤维化大鼠的预防作用。方法采用CCl4诱导大鼠肝纤维化模型,TFLC灌胃给药第12周末,检测ALT、AST及HA、LN、PⅢNP、CⅣ含量;检测Ⅰ型胶原(CollagenⅠ)、瘦素、TGF-β1的含量;检测Hyp及组织病理变化;检测瘦素受体(OB-Rb)、TGFβ1Ⅰ型受体(TGFβR1)、Smad3 mRNA及蛋白表达。结果 TFLC(200、400 g.L-1)能明显降低肝纤维化大鼠肝纤维化指标及血清Ⅰ型胶原、瘦素、TGF-β1水平;降低Ob-Rb、TGFβR1、Smad3的mRNA及蛋白表达。结论 TFLC能有效预防CCl4诱导的大鼠肝纤维化,可能与下调肝内瘦素及其受体,抑制TGF-β1/Smad通路有关。Aim To evaluate whether Litsea Coreana level（TFLC） would have regulatory effects on the expressions of Leptin and TGF-β1 of hepatic fibrosis in rats.Methods The liver fibrosis model rats were in duced by injecting 50% CCl 4（1 ml.kg-1） twice a week for 12 weeks.From the first week,all the therapeutic groups were treated with TFLC（100,200,400 mg.kg-1） and the colchicines（0.1 mg.kg-1） respectively.At the end of the twelfth week,the levels of ALT,AST,HA,LN,P Ⅲ NP,CIV,Collagen Ι,Leptin,TGF-β1 in serum and Hyp in liver were measured.Masson staining was used to evaluate the degree of hepatic fibrosis.The mRNA and protein expressions of Ob-Rb,TGF-βR1 and Smad3 in liver were detected by RT-PCR and Western blot.Results TFLC（200 and 400 mg.kg-1） treatment significantly reduced the elevations of ALT,AST,HA,LN,PⅢNP,CIV,Collagen Ⅰ,Leptin,TGF-β1 in serum and Hyp in liver.In addition,TFLC treatment improved the morphologic changes of hepatic fibrosis,suppressed expressions of Ob-Rb,TGF-βR1,Smad3 in the liver fibrosis in rats.Conclusions TFLC is able to ameliorate liver injury and protect rats from liver fibrosis.This process may be related to inhibiting the expression of Ob-Rb,TGFβR1 and Smad3.

关 键 词：肝纤维化 豹皮樟总黄酮 瘦素 瘦素受体 转化生长因子-Β1 TGF-β1Ⅰ型受体 

分 类 号：R575.2[医药卫生—消化系统]