基因p16及CyclinD_1在子宫内膜癌中的表达及意义  

Expression of p16 and Cyclin D_1 in uterine endometrial carcinomas and its significance

作　　者：贺书云[1] 辛晓燕[1] 王健[1] 郭惠玲[1] 

机构地区：[1]第四军医大学西京医院妇产科,陕西西安710033

出　　处：《第四军医大学学报》2000年第3期347-349,共3页Journal of the Fourth Military Medical University

摘　　要：目的　探讨抑癌基因 p16及细胞周期素 D1 (CyclinD1 )在子宫内膜癌、交界性子宫内膜组织及正常子宫内膜组织中的表达规律及其与子宫内膜癌的关系 .方法　应用免疫组化 SABC法检测 38例子宫内膜癌 ,19例子宫内膜增生过长及不典型增生 ,10例正常子宫内膜组织中基因 p16 ,CyclinD1 的表达 .结果　 p16在子宫内膜癌组织中阳性率为 40 % ,在交界性子宫内膜组织中表达阳性率为 6 8% ,在正常子宫内膜组织中无表达 ,正常、交界性及恶性子宫内膜组织中表达阳性率有明显差异 (P<0 .0 5 ) .在子宫内膜癌组织中 p16的表达与病理分级有关 ,与临床分期无明显关联 .Cyclin D1 在子宫内膜癌中呈明显阳性表达 ,表达率 5 7% ,定位于细胞核 ,在交界性子宫内膜病变中阳性率为 2 1% ,正常子宫内膜组织中无表达 ,正常、交界性及恶性子宫内膜组织表达差异明显 (P<0 .0 5 ) .在子宫内膜癌组织中 Cyclin D1 的表达与临床分期及病理分级均无关 ,但有随着分化程度的下降、临床期别的升高而升高的趋势 .结论　 p16 ,Cyclin D1 在子宫内膜癌的发生发展过程中具有协同作用 .AIM To study the expression and significance of p16 and Cyclin D 1 in human uterine endometrial carcinomas. METHODS The expression of p16 and Cyclin D 1 was exa mined in malignant, borderline and benign uterine endometrial tissues by immunohistochemistry SABC methods. Monoclonal antibodies against p16 and Cyclin D 1 were employed. RESULTS The positive rates of p16 expression among the malignant, borderline and benign tumors were 40%, 68% and 0 respectively. Significant difference was observed ( P <0.05) in malignant tumors. The expression of p16 was associated with pathological grades and no association was found with clinical phases. Cyclin D 1 expression was negative in benign tumors and the positive rates in malignant and borderline tumors were 53% and 21% respectively ( P <0.05). In uterine endometrial carcinomas, no association was found between clinical phases and pathological grades. CONCLUSION There is an overexpression of Cyclin D 1 and a lower expression of p16 in uterine endometrial carcinomas and the synergy of the expression of p16 and Cyclin D 1 may contribute to the development of uterine endometrial carcinomas.

关 键 词：子宫内膜癌 P16 CYCLIND1 免疫组织化学 

分 类 号：R737.330.2[医药卫生—肿瘤]