66例氨基糖甙类抗生素致聋患者的线粒体DNA1555位突变检测  

Detection of Mitochondrial DNA Mutation at nt1555 in 66 Patients with Aminoglycoside Antibiotic-induced Deafness

作　　者：陈观明[1] 傅四清[2] 滕云[2] 葛新[1] 王晓珊 胡晓峰[2] 崔永华[1] 

机构地区：[1]同济医科大学附属同济医院耳鼻喉科,武汉430030 [2]同济医科大学医学遗传研究室,武汉430030

出　　处：《同济医科大学学报》2000年第2期149-150,153,共3页Acta Universitatis Medicinae Tongji

摘　　要：目前认为 mt DNAA15 5 5 G突变与氨基糖甙类抗生素致聋 (AAID)有关。通过调查采集了 6 6例 AAID病例 ,采外周血作 PCR- RFL P分析 ,对照组为 30例正常人 ,分析 mt DNAA15 5 5 G突变在 AAID中的作用。研究发现 :6 6例AAID患者中 ,14例具有 A15 5 5 G突变 ,而其余的 5 2例和对照组均无此突变。这表明 mt DNAA15 5 5 G突变是人体对氨基糖甙类抗生素遗传易感性的分子基础之一 ,基因和环境因素的相互作用可能在 AAID的发生中起重要作用。Sixty cases of aminoglycoside antibiotics induced deafness (AAID) were selected. The peripheral blood was taken from the patients to be subjected to PCR RFLP assay. Thirty normal subjectes served as control group. The role of mtDNAA1555G mutation in the pathogenesis of AAID was studied. The A1555G mutation was found in 14 deaf patients but not in the remaining 52 patients or in the 30 control subjects. These data indicate that the A1555G mutation is one of the molecular basis for human hypersensitivity to aminoglycoside antibiotics. It is likely that the interaction of genes (nuclear and mitochondria) with environmental factor plays an important role in the development of deafness.

关 键 词：氨基糖甙类 抗生素 致聋 线粒体DNA 突变 耳聋 

分 类 号：R978.12[医药卫生—药品] R764.430.2[医药卫生—药学]