细胞角蛋白基因13在鼻咽癌中的表达研究  

Cytokeratin 13 gene expression in human nasopharyngeal carcinoma

作　　者：邱元正[1] 田勇泉[1] 肖健云[1] 陶正德[1] 赵素萍[1] 李桂源[2] 

机构地区：[1]湖南医科大学湘雅医院耳鼻喉科,长沙410008 [2]湖南医科大学肿瘤研究所

出　　处：《中华肿瘤杂志》2000年第2期129-131,共3页Chinese Journal of Oncology

基　　金：国家863高技术计划经费资助!项目 (1 0 2 1 0 0 1 0 5)

摘　　要：目的　探讨细胞角蛋白基因 13(Cytokeratin 13,CK13)在人鼻咽癌 (NPC)中的表达。方法应用免疫组化检测了 40例NPC组织和 8例慢性鼻咽炎 (CINE)组织中CK13蛋白水平的表达 ,同时应用Northern杂交对其中 32例NPC和 8例CINE组织进行了mRNA水平检测 ,并分析了CK13表达与NPC临床特征的关系。结果　在NPC中 ,CK13在蛋白质水平和mRNA水平均较CINE显著降低 (P <0 0 1) ,且CK13表达与NPC颈淋巴结转移有显著相关性 (P <0 0 5 ) ,与肿瘤的T分期无显著相关性(P >0 0 5 )。结论　CK13基因的表达可能与NPC组织分化以及颈淋巴结转移有关。Objective To investigate the possible pathogenetic role of cytokeratin 13 (CK13) gene expression in human nasopharyngeal carcinoma (NPC).Methods Tissue samples taken from a total of 40 cases of NPC and 8 cases of chronic inflammation of nasopharyngeal epithelium (CINE) were immunohistochemically studied for CK13 protein expression. Northern blot hybridization was used to detect CK13 gene at mRNA level in 32 of the 40 NPC and in 8 CINE cases. Correlations of CK13 expression with the clinical features of NPC were also investigated.Results Significantly stronger immunoreactivity for CK13 protein was shown in CINE than in NPC tissues ( P <0.01), so did it at mRNA levels. Significantly higher CK13 expression was demonstrated in patients with cervical lymph node metastasis, as compared to those without ( P <0.05). However, no significant correlation between CK13 expression and clinical staging of the disease.Conclusion The decreased expression of CK13 in NPC suggests that differentiation disturbance of the nasopharyngeal epithelium might play a role in the pathogenesis of NPC. Subject

关 键 词：鼻咽肿瘤 CKB 基因表达 免疫组织化学 

分 类 号：R739.63[医药卫生—肿瘤]