大肠癌细胞周期调控因子表达与微卫星不稳定性的关系  

Relationship between the expression of cell cycle regulators and microsatellite instability in colorectal carcinomas

作　　者：阎晓初[1] 柳凤轩[1] 房殿春[2] 罗元辉[2] 鲁荣[2] 

机构地区：[1]重庆第三军医大学附属西南医院病理科,400038 [2]重庆第三军医大学附属西南医院消化内科,400038

出　　处：《中华肿瘤杂志》2000年第2期141-144,共4页Chinese Journal of Oncology

摘　　要：目的　探讨大肠癌组织中细胞周期调控因子表达与微卫星不稳定性 (MSI)的关系。方法　采用免疫组化、PCR SSLP法对 5 6例大肠癌分别进行细胞周期调控因子CyclinD1、P16、P2 1WAF1/CIP1、P5 3、Rb以及PCNA的观测和D18S34、D17S799、D5S40 9、TCF 2、P5 3和Rb 6个碱基序列位点MSI检测。结果　大肠癌MSI总阳性率 44 .6 % ,其中 1个位点阳性 7例 ,≥ 2个位点阳性 18例 ,占32 .1%。MSI阳性 (MSI+ )大肠癌CyclinD1、P5 3蛋白阳性表达率显著低于MSI阴性 (MSI-)大肠癌 ,而P2 1蛋白阳性表达率显著高于MSI-肿瘤组 (P <0 .0 5 ) ,MSI+ 大肠癌P16阳性率也较MSI-组略增高 ,MSI+ 和MSI-大肠癌Rb蛋白阳性表达率较接近。结论　P2 1蛋白过度表达对大肠癌MSI的发生可能具有较重要的促进作用 ,CyclinD1、P5 3蛋白表达减少或P16蛋白过度表达与大肠癌MSI的发生可能也有关。Objective To explore the relationship between the expression of cell cycle regulators and microsatellite instability (MSI) in colorectal carcinomas(CRC).Methods The expression of cell cycle regulators cyclin D1, P16, P21 WAF1/CIP1 , P53, Rb and PCNA were detected by immunohistochemical staining, and MSI at six microsatellite loci (D18S34, D17S799, D5S409, TCF 2, Rb and P53) were examined by PCR SSLP in 56 cases of colorectal carcinomas.Results The overall positive rate of MSI of CRC was 44.6% (25/56). MSI was positive at 1 locus in 7 cases, at 2 and more than 2 loci in 18 cases. In CRC with MSI, the positive rate of cyclin D1 and P53 protein was significantly lower, while that of P21 protein was significantly higher than those without MSI ( P <0.05). The positive rate of P16 in CRC with MSI was higher than those without MSI. The expression of Rb proteins was similar in frequency in CRC with or without MSI.Conclusion Overexpression of P21 may play an important role in MSI genesis in CRC. It may also be related to decreased expression of cyclin D1 and P53 and overexpression of P16. Subject

关 键 词：大肠癌 细胞周期 微卫星不稳定性 

分 类 号：R735.34[医药卫生—肿瘤]