Determination of Quinolone Antibiotics in Water Using Solid Phase Extraction-High Performance Liquid Chromatography-Fluorescence Method  

作　　者：WANG Qiao-jun YI Ru-han MO Ce-hui 

机构地区：[1]Guangzhou Yutong Enterprise Management Consultation Co., Ltd., Guangzhou 510632, China [2]Department of Environmental Engineering, Jinan University, Guangzhou 510632, China

出　　处：《Animal Husbandry and Feed Science》2011年第2期45-48,共4页动物与饲料科学（英文版）

基　　金：funded by the grants from the China Natural Science Foundation ( 30671208 and 40773062);Key Project of Guangdong Natural Science Foundation ( 07117909);Science and Technology Planning Project of Guangdong Province( 2005B20801002 and 2006B20601003)

摘　　要：[Objective] To develop a solid phase extraction-high performance liquid chromatography-fluorescence method for determination of quin- olone antibiotics in water. [ Metbod] The standard curves of four quinolones （norfloxacin, ciprofloxacin, Iomefloxacin and enrofloxacin） were pre- pared. The detection limit in water and recovery were determined. The water samples collected from different areas, river and tap water were trea- ted using solid-phese extraction method and analyzed by high performance liquid chromatography. Then the concentration of quinolones antibiotics was determined by fluorescence method. [ Result] The detection limit of quinolone antibiotics in water was 0.083 -0.248 μg/L, and their recovery was 63.7% -134.1%. The four quinolone antibiotics at different levels were detected in various water samples, and the total concentration of quin- olone antibiotics was 0.045 -3.969 μg/L. The total concentration of quinolone antibiotics was higher in the water samples collected from rivers in Shenzhen area than in the sewage samples. The four quinolone antibiotics could be detected in all tap water samples. [ CoaduLsion ] The solid phase extraction-high performance liquid chromatography-fluorescence method is feasible and effective to detect quinolones in water. In addition, this method needs low cost and can meet requirements of daily monitorina and analysis.[Objective] To develop a solid phase extraction-high performance liquid chromatography-fluorescence method for determination of quin- olone antibiotics in water. [ Metbod] The standard curves of four quinolones （norfloxacin, ciprofloxacin, Iomefloxacin and enrofloxacin） were pre- pared. The detection limit in water and recovery were determined. The water samples collected from different areas, river and tap water were trea- ted using solid-phese extraction method and analyzed by high performance liquid chromatography. Then the concentration of quinolones antibiotics was determined by fluorescence method. [ Result] The detection limit of quinolone antibiotics in water was 0.083 -0.248 μg/L, and their recovery was 63.7% -134.1%. The four quinolone antibiotics at different levels were detected in various water samples, and the total concentration of quin- olone antibiotics was 0.045 -3.969 μg/L. The total concentration of quinolone antibiotics was higher in the water samples collected from rivers in Shenzhen area than in the sewage samples. The four quinolone antibiotics could be detected in all tap water samples. [ CoaduLsion ] The solid phase extraction-high performance liquid chromatography-fluorescence method is feasible and effective to detect quinolones in water. In addition, this method needs low cost and can meet requirements of daily monitorina and analysis.

关 键 词：Solid-phase extraction High performance liquid chromatography WATER Quinolone antibiotics 

分 类 号：X132[环境科学与工程—环境科学] TQ465[化学工程—制药化工]