Determination of the Activity of Acetylcholinesterase(AChE) in Pardosa astrigera and the Inhibition Effects of Pesticides on Its Activity  

星豹蛛乙酰胆碱酯酶(AChE)活性测定及药剂对其活性的抑制作用(英文)

作　　者：马敏[1] 张宾[2] 葛衍珍[1] 李生才[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]山西农业大学文理学院,山西太谷030801

出　　处：《Plant Diseases and Pests》2011年第1期60-63,共4页植物病虫害研究（英文版）

基　　金：Supported by Shanxi Scholarship Fund(2009042);Youth Science andTechnology Research Fund in Shanxi Province(2008021041)~~

摘　　要：[ Objective ] The paper was to systemically study the characteristics of acetyl cholinesterase （ACHE） in Pardosa astrigera, and confirm the occurrence and development of its pesticide resistance and the resistance level, thereby establishing a quick and accurate detection method for enzyme activity. [ Method] The optimal conditions for assaying the activity of AChE in different parts of P. astrigera were determined by orthogonal experiment. The distribution conditions of acetyl cholinesterase （AChE） in different tissues of P. astrigera were further studied, and the sensitivities of the enzyme tO four common pesticides were also determined. [ Result] The optimal condition for assaying the activity of AChE in cephalothorax, abdomen and appendage of P. astrigera was as follows, enzyme concentrations： 12, 18 and 29 g/L; substrate concentrations： 0.6, 1.0 and 1.0 mmol/L; pH value, 7.0; reaction temperatures： 30, 35, 35℃ ; reaction time, 5 rain. AChE was mainly distributed in the cephalothorax of P. astrigera. The specific activity of AChE in the extract solution with Triton X-100 was higher than that in the solution without Triton X-100. The median inhibitory concentrations （IC50） of methomyl, phoxim, betacypermethrin, chlorpyrifos against AChE in the cephalothorax of P. astrigera were 7.76 × 10^-5, 1.76×10^-4, 4.12 ×10^-4 and 4.94 ×10^-4 mol/L, respectively. [ Conclusion] AChEs in P. astrigera were membrane-bounded. The inhibition of four pesticides against AChE in the cephalothorax of P. astrigera had good dese-effect, this indicated that AChE in the cephalothorax of P. astrig- era could be used as the biochemical marker to monitor the contamination of organic phosphorus, carbamate and pyrethroid pesticides in the environment.[ Objective ] The paper was to systemically study the characteristics of acetyl cholinesterase （ACHE） in Pardosa astrigera, and confirm the occurrence and development of its pesticide resistance and the resistance level, thereby establishing a quick and accurate detection method for enzyme activity. [ Method] The optimal conditions for assaying the activity of AChE in different parts of P. astrigera were determined by orthogonal experiment. The distribution conditions of acetyl cholinesterase （AChE） in different tissues of P. astrigera were further studied, and the sensitivities of the enzyme tO four common pesticides were also determined. [ Result] The optimal condition for assaying the activity of AChE in cephalothorax, abdomen and appendage of P. astrigera was as follows, enzyme concentrations： 12, 18 and 29 g/L; substrate concentrations： 0.6, 1.0 and 1.0 mmol/L; pH value, 7.0; reaction temperatures： 30, 35, 35℃ ; reaction time, 5 rain. AChE was mainly distributed in the cephalothorax of P. astrigera. The specific activity of AChE in the extract solution with Triton X-100 was higher than that in the solution without Triton X-100. The median inhibitory concentrations （IC50） of methomyl, phoxim, betacypermethrin, chlorpyrifos against AChE in the cephalothorax of P. astrigera were 7.76 × 10^-5, 1.76×10^-4, 4.12 ×10^-4 and 4.94 ×10^-4 mol/L, respectively. [ Conclusion] AChEs in P. astrigera were membrane-bounded. The inhibition of four pesticides against AChE in the cephalothorax of P. astrigera had good dese-effect, this indicated that AChE in the cephalothorax of P. astrig- era could be used as the biochemical marker to monitor the contamination of organic phosphorus, carbamate and pyrethroid pesticides in the environment.

关 键 词：Pardosa astrigera ACETYLCHOLINESTERASE Biochemical marker Sensitivity to pesticide IC50 

分 类 号：S186[农业科学—农业基础科学]