Development of Multiplex-PCR for Identification of Pasteurella multocida,Haemophilus parasuis and Actinbacillus pleuropneumoniae  

作　　者：HE Ying,ZHAO Ping,CHU Yue-feng,GAO Peng-cheng,LU Zhong-xin Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences State Key Laboratory of Veterinary Etiological Biology Key laboratory of Grazing Animal Diseases of Ministry of Agriculture Key Laboratory of Animal Virology of Ministry of Agriculture,Lanzhou 730046,China 

出　　处：《Animal Husbandry and Feed Science》2010年第8期25-27,共3页动物与饲料科学（英文版）

基　　金：funded by the subproject of National Key Technology R&D Program (2006BAD06A01 and 2006BAD06A12);Basic Work of National Science and Technology Special Plan (2008FY210200)

摘　　要：[ Objective] To develop multiplex-PCR for diagnosis of mixed infection caused by Pasteurella multocida （ PM）, Haomophilus parasuis （HPS） and Actinbaci/lus pleuropneumoniae （App）. [ Method ] PCR method was developed to detect single infection caused by PM, HPS or App. The conditions of amplification and primers were optimized, and the multiple-PCR was developed to detect mixed infection of PM, HPS and App. [ Result] A 457-bp band, a 821-bp band and a 342-bp band were simultaneously amplified in the one PCR reaction system. The method had high sensitivity and specificity. [ Conduslon] The multiple-PCR is successfully developed and can be used for differential diagnosis of PM, HPS and App.[ Objective] To develop multiplex-PCR for diagnosis of mixed infection caused by Pasteurella multocida （ PM）, Haomophilus parasuis （HPS） and Actinbaci/lus pleuropneumoniae （App）. [ Method ] PCR method was developed to detect single infection caused by PM, HPS or App. The conditions of amplification and primers were optimized, and the multiple-PCR was developed to detect mixed infection of PM, HPS and App. [ Result] A 457-bp band, a 821-bp band and a 342-bp band were simultaneously amplified in the one PCR reaction system. The method had high sensitivity and specificity. [ Conduslon] The multiple-PCR is successfully developed and can be used for differential diagnosis of PM, HPS and App.

关 键 词：Haemophilus parasuis Pasteurella multocida Actinbacillus pleuropneumoniae MULTIPLEX-PCR 

分 类 号：S858.28[农业科学—临床兽医学] TS207.3[农业科学—兽医学]