MULTIPLEX-PCR

作品数：5被引量：6H指数：2
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相关领域：农业科学医药卫生更多>>
相关作者：孟庆鹏李琳李雪娟更多>>
相关机构：南京农业大学第四军医大学口腔医院陕西师范大学更多>>
相关期刊：《现代生物医学进展》《World Journal of Clinical Cases》《Journal of Integrative Agriculture》《Advances in Bioscience and Biotechnology》更多>>
相关基金：国家科技支撑计划国家自然科学基金更多>>
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Hepatitis B virus in cerebrospinal fluid of a patient with purulent bacterial meningitis detected by multiplex-PCR:A case report被引量：2
《World Journal of Clinical Cases》2022年第5期1697-1701,共5页Dai-Quan Gao Yong-Qiang Hu Xin Wang Yun-Zhou Zhang 
BACKGROUND Bacterial meningitis(BM)is a common central nervous system inflammatory disease.BM may cause serious complications,and early diagnosis is essential to improve the prognosis of affected patients.CASE SUMMARY...
关键词：Purulent meningitis Streptococcus pneumoniae Hepatitis B virus Multiplex PCR Cerebrospinal fluid Case report 
Comparative genomics provide a rapid detection of Fusarium oxysporum f. sp. conglutinans被引量：1
《Journal of Integrative Agriculture》2016年第4期822-831,共10页LING Jian ZHANG Ji-xiang ZENG Feng CAO Yue-xia XIE Bing-yan YANG Yu-hong 
supported in part by the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, P.R.China;the National Natural Science Foundation of China (31571962, 31272003);the National Key Technology R&D Program of China (2012BAD19B06);the Special Fund for Agro-Scientific Research in the Public Interest, China (200903049-04);the National Staple Vegetable Industry Technology System Construction Project, China (CARS-25-B-01);the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences
Fusarium oxysporum f. sp. conglutinans （Foc） is the causal agent of Fusarium wilt disease of Brassica oleracea. A rapid, accurate, and reliable method to detect and identify plant pathogens is vitally important to i...
关键词：Fusarium oxysporum wilt disease CABBAGE MULTIPLEX-PCR molecular detection comparative genomics 
复合PCR扩增人mtDNA HVR方法的初探被引量：1
《现代生物医学进展》2013年第7期1360-1363,共4页李琳 邵金陵 段小红 李雪娟 刘振霞 
国家自然科学基金项目(31070835);陕西省社发公关课题基金项目(2011k15-06-04)
目的:建立一种高效的扩增线粒体DNA高可变区(mtDNA HVR)的方法。方法:本研究选取5例健康成人静脉血,用人血全基因组DNA试剂盒,提取全基因组DNA,设计引物,用复合PCR方式,对线粒体DNA中的高可变区进行扩增。复合扩增的方式为:用6对套叠引...
关键词：MTDNA HVR MULTIPLEX-PCR 套叠引物 
Evaluation of an internal control in a multiplex-PCR assay for sex determination of <i>in vitro</i>-produced bovine embryos
《Advances in Bioscience and Biotechnology》2011年第6期456-462,共7页Fabiana de Almeida Rufino Marcelo Marcondes Seneda Alice Fernandes Alfieri Katia Cristina Silva-Santos Karina Cristina Puggesi Rubin Amauri Alcindo Alfieri 
The use of an internal control in a multiplex-PCR assay for sex determination of In Vitro-produced bovine embryos was evaluated in biopsies of groups of 54 fresh and 44 frozen embryos. The internal controls used were ...
关键词：BOVINE Embryos In VITRO Production SEXING MULTIPLEX-PCR 
Development of Multiplex-PCR for Identification of Pasteurella multocida,Haemophilus parasuis and Actinbacillus pleuropneumoniae被引量：2
《Animal Husbandry and Feed Science》2010年第8期25-27,共3页HE Ying,ZHAO Ping,CHU Yue-feng,GAO Peng-cheng,LU Zhong-xin Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences State Key Laboratory of Veterinary Etiological Biology Key laboratory of Grazing Animal Diseases of Ministry of Agriculture Key Laboratory of Animal Virology of Ministry of Agriculture,Lanzhou 730046,China 
funded by the subproject of National Key Technology R&D Program (2006BAD06A01 and 2006BAD06A12);Basic Work of National Science and Technology Special Plan (2008FY210200)
[ Objective] To develop multiplex-PCR for diagnosis of mixed infection caused by Pasteurella multocida （ PM）, Haomophilus parasuis （HPS） and Actinbaci/lus pleuropneumoniae （App）. [ Method ] PCR method was develo...
关键词：Haemophilus parasuis Pasteurella multocida Actinbacillus pleuropneumoniae MULTIPLEX-PCR 
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