Ultraviolet Mutagenesis of Bacillus licheniformis LCB-8 and Screening of High-Yielding Strain  

作　　者：LV Zhi-wei WANG Jin ZHANG Wen-hui 

机构地区：[1]School of Life Sciences, Liaocheng University, Liaocheng 252059, China [2]School of Architecture & Civil Engineering, Liaocheng Universi-ty, Liaocheng 252059, China

出　　处：《Animal Husbandry and Feed Science》2011年第6期19-20,22,共3页动物与饲料科学（英文版）

基　　金：supported by the grants from Initial Scientific Research Foundation for Doctors in Liaocheng University

摘　　要：[ Objective ] To enhance amylase, protease and cellulase activities of Bacillus licheniformis LCB-8 by ultraviolet mutagenasis. [ Method ] Ultraviolet light was used to mutate this strain. The activities of amylase, protease and cellulase were determined to get high-yielding strains. [ Resalt] The best time for ultraviolet mutation was 30 s, and the mortality rate reached 72.4%. After primary screening and rascraening, two better strains were obtained. After mutagenasis, the activities of amylase, protease and cellulase of DY-97 strain were enhanced by 150%, 78% and 17% respectively. After mutagenesis, the activities of cellulase, amylase and protease of DY-34 strain were enhanced by 31%, 46% and 64% respectively. [ Conclusion] The DY-97 strain is suitable for fermentation of feed rich in protein and amylum, whereas the DY-34 strain is suitable for fermentation of feed rich in cellulose.[ Objective ] To enhance amylase, protease and cellulase activities of Bacillus licheniformis LCB-8 by ultraviolet mutagenasis. [ Method ] Ultraviolet light was used to mutate this strain. The activities of amylase, protease and cellulase were determined to get high-yielding strains. [ Resalt] The best time for ultraviolet mutation was 30 s, and the mortality rate reached 72.4%. After primary screening and rascraening, two better strains were obtained. After mutagenasis, the activities of amylase, protease and cellulase of DY-97 strain were enhanced by 150%, 78% and 17% respectively. After mutagenesis, the activities of cellulase, amylase and protease of DY-34 strain were enhanced by 31%, 46% and 64% respectively. [ Conclusion] The DY-97 strain is suitable for fermentation of feed rich in protein and amylum, whereas the DY-34 strain is suitable for fermentation of feed rich in cellulose.

关 键 词：Bacillus Iichenfformis Ultraviolet mutation Enzyme activity 

分 类 号：TQ921.7[轻工技术与工程—发酵工程] Q939.124[生物学—微生物学]