Determination of Endophytic Bacteria Resistance against Rifampin and UV-mutagenesis  

植物内生细菌对利福平的抗性及紫外诱变研究(英文)

作　　者：刘佳佳[1] 马竹凤[1] 闫淑珍[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210046

出　　处：《Plant Diseases and Pests》2010年第3期25-27,共3页植物病虫害研究（英文版）

基　　金：Supported by Basic Science Talent Training Fund of Nanjing Normal University(J0730650)~~

摘　　要：The study aimed to isolate and screen endophytic bacteria from the plant in order to understand their colonization dynamics in plants. [Method] 5 kinds of endophytic bacteda including H1, DP1, CJL1, DJL12 and YC1 were selected as the original strains, and conducted UV mutagenesis studies on H1 and DJL12 with weak resistance, while the resistance of them against rifampicin was confirmed. [Result] Through preliminary screening, five kinds of endophytic bacteria could grew well in the plate without rifampin, among them, H1 and DLJ12 were unable to survive under 10 μg/ml concentration of rifampicin, and the other three strains could survive under 50 μg/ml concentration of rifampicin. After UV mutagenesis, DLJ12 strain with dfampicin resistance concentration of 80 μg/ml was obtained. [ Conclusion] The study could provide theoretical and practical basis for development and utilization of endophytic bacteria.The study aimed to isolate and screen endophytic bacteria from the plant in order to understand their colonization dynamics in plants. [Method] 5 kinds of endophytic bacteda including H1, DP1, CJL1, DJL12 and YC1 were selected as the original strains, and conducted UV mutagenesis studies on H1 and DJL12 with weak resistance, while the resistance of them against rifampicin was confirmed. [Result] Through preliminary screening, five kinds of endophytic bacteria could grew well in the plate without rifampin, among them, H1 and DLJ12 were unable to survive under 10 μg/ml concentration of rifampicin, and the other three strains could survive under 50 μg/ml concentration of rifampicin. After UV mutagenesis, DLJ12 strain with dfampicin resistance concentration of 80 μg/ml was obtained. [ Conclusion] The study could provide theoretical and practical basis for development and utilization of endophytic bacteria.

关 键 词：Endophytic bacteria UV mutagenesis RIFAMPICIN 

分 类 号：S476[农业科学—农业昆虫与害虫防治]