Polymorphism of Intron 14 of Porcine CACNA2D1 Gene and Effects on Muscular pH_(45)  

CACNA2D1基因第14内含子的多态性及其对猪肌肉pH_(45)的影响

作　　者：李建华[1] 吕帮玉[1] 杨明生[1] 

机构地区：[1]孝感学院生命科学技术学院,湖北孝感432003

出　　处：《Animal Husbandry and Feed Science》2009年第2期1-4,共4页动物与饲料科学（英文版）

基　　金：湖北省教育厅优秀中青年人才项目(Q200626001)~~

摘　　要：[ Objective] To detect polymorphism of intron 14 of porcine CACNA2Dt gene and thus provide conditions for the marker-assisted selec- tion of pork quality traits. [Method] The polymorphism of the exon 14 and intron 14 of porcine CACNA2D1 gene was detected in six breeds by PCR- SSCP. The genetic effects of different genotypes on the pH45 value of eye muscle and ham joint muscle were also analyzed. I-Result] There were three kinds of genotypes, namely, AA, AG and GG, in the intron 14 of porcine CACNA2 DI gene. The polymorphic locus （ G --~ A） was at the 1 145 nt of the sequence （GenBank Accepted No. FJ156361 ）. The results of X2 test showed that the distribution of these three kinds of genotypes was significantly different in the six breeds （P〈0.05）. The pH45 values of eye muscle and ham joint muscle were significantly different between geno- type AA and genotype AG of pigs in F2 generation from the Jianhua x Pietrain resource family （ P 〈 0.05）. [Conclusion ] The polymorphism of the in- tron 14 of porcine CACNA2D1 gene has effects on the pH45 values of eye muscle and ham joint muscle.[ Objective] To detect polymorphism of intron 14 of porcine CACNA2Dt gene and thus provide conditions for the marker-assisted selec- tion of pork quality traits. [Method] The polymorphism of the exon 14 and intron 14 of porcine CACNA2D1 gene was detected in six breeds by PCR- SSCP. The genetic effects of different genotypes on the pH45 value of eye muscle and ham joint muscle were also analyzed. I-Result] There were three kinds of genotypes, namely, AA, AG and GG, in the intron 14 of porcine CACNA2 DI gene. The polymorphic locus （ G --~ A） was at the 1 145 nt of the sequence （GenBank Accepted No. FJ156361 ）. The results of X2 test showed that the distribution of these three kinds of genotypes was significantly different in the six breeds （P〈0.05）. The pH45 values of eye muscle and ham joint muscle were significantly different between geno- type AA and genotype AG of pigs in F2 generation from the Jianhua x Pietrain resource family （ P 〈 0.05）. [Conclusion ] The polymorphism of the in- tron 14 of porcine CACNA2D1 gene has effects on the pH45 values of eye muscle and ham joint muscle.

关 键 词：PIG CACNA2D1 gene PCR-SSCP pH45 

分 类 号：S828[农业科学—畜牧学]