Developmental Changes of GHRmRNA Expression Level in Sheep Liver  

绵羊肝脏生长激素受体基因表达的发育性变化研究

作　　者：黄治国[1,2] 谢庄[2] 

机构地区：[1]四川理工学院酿酒生物技术及应用四川省重点实验室,四川自贡643000 [2]南京农业大学动物科技学院,江苏南京210095

出　　处：《Animal Husbandry and Feed Science》2009年第2期8-12,共5页动物与饲料科学（英文版）

基　　金：新疆生产建设兵团博士基金(2003-02)~~

摘　　要：[Objective] To detect the expression of liver growth hormone receptor gene （GHR） during the eady development of Kazak sheep and Xinjiang fine-wool sheep, and thus provide information for the research about the early growth and development of sheep. [ Method] With real-time quantitative PCR, the liver GHR mRNA level was separately detected in two varieties of sheep at 2, 30, 60, 90 and 120 days old. Then the data was analyzed with SPSS software. E Result] The liver GHR gene was highly expressed in 2 day-old sheep, but the expression level fell to the lowest point at 30 days old and then rose continuously; the change trend after 30 days old was positively correlated with the cumulative growth curves of sheep （ P 〈0.05） ; GHR mRNA level was lower in Kazak sheep than in Xinjiang fine-wool sheep during 2 -90 days old, but the difference was ex- tremely significant （P 〈0.01 ） only at 2 days old and 90 days old. [ Conclusion] The male Kazak sheep and Xinjiang fine-wool sheep have similar model of developmental changes of liver GHR mRNA and the differences are smaller between these two species; GHR gene may play an important role in the regulation of sheep growth.[Objective] To detect the expression of liver growth hormone receptor gene （GHR） during the eady development of Kazak sheep and Xinjiang fine-wool sheep, and thus provide information for the research about the early growth and development of sheep. [ Method] With real-time quantitative PCR, the liver GHR mRNA level was separately detected in two varieties of sheep at 2, 30, 60, 90 and 120 days old. Then the data was analyzed with SPSS software. E Result] The liver GHR gene was highly expressed in 2 day-old sheep, but the expression level fell to the lowest point at 30 days old and then rose continuously; the change trend after 30 days old was positively correlated with the cumulative growth curves of sheep （ P 〈0.05） ; GHR mRNA level was lower in Kazak sheep than in Xinjiang fine-wool sheep during 2 -90 days old, but the difference was ex- tremely significant （P 〈0.01 ） only at 2 days old and 90 days old. [ Conclusion] The male Kazak sheep and Xinjiang fine-wool sheep have similar model of developmental changes of liver GHR mRNA and the differences are smaller between these two species; GHR gene may play an important role in the regulation of sheep growth.

关 键 词：SHEEP LIVER Growth hormone receptor gene EXPRESSION 

分 类 号：S826[农业科学—畜牧学]