甲异靛对白血病骨髓基质细胞调节白血病细胞增殖的影响  

Effect of meisoindigo on proliferation of leukemic cells regulated by bone marrow stromal cells

作　　者：王一[1] 连小赟[1] 高瑛[1] 张玎[1] 王晖[1] 王岐山[1] 

机构地区：[1]陕西省人民医院血液科,西安710068

出　　处：《白血病．淋巴瘤》2012年第11期668-670,共3页Journal of Leukemia & Lymphoma

基　　金：陕西省自然科学基金（2010JM4018）

摘　　要：目的探讨甲异靛对白血病骨髓基质细胞干预白血病细胞增殖的影响。方法利用白血病骨髓单个核细胞培养骨髓基质细胞，并建立白血病细胞和骨髓基质的共培养体系。用锥虫蓝拒染实验测定甲异靛对共培养体系中白血病细胞增殖的影响；流式细胞术检测白血病细胞膜上CXCR4的表达。结果白血病骨髓基质细胞可抑制白血病细胞的增殖。低浓度的甲异靛（5txmol／L）可促进白血病和骨髓基质细胞共培养体系中白血病细胞的增殖。白血病细胞膜异常高表达CXCR4，甲异靛可明显抑制HL-60细胞和原代白血病细胞膜上CXCR4的表达，骨髓基质细胞可以促进白血病细胞膜上CXCR4的表达。结论甲异靛可能通过下调白血病细胞膜上CXCR4的表达起抗白血病作用。Objective To observe the effect of meisoindigo on co-culture leukemic cells and bone marrow stromal cells. Methods Bone marrow stromal cells were cultured using bone marrow mononuclear cells from patients with leukemia, and built the co-culture leukemic cells and bone marrow stromal ceils. Trypan-blue-exclusion test detected the proliferation of leukemic cells. The expression levels of membrane CXCR4 on leukemic cells were assessed by flow cytometric analysis. Results Bone marrow stromal cell of leukemia could inhibit leukemic cells proliferation, leukemic cells could be promoted their growth when exposed to low concentration （5 p^mol/L） of meisoindigo. Abnormal high expression of CXCR4 in leukemic cells, and meisoindigo could markedly inhibit the expression of CXCR4 in HL-60 and del leukemic cells. Expression of CXCR4 in leukemic cells can up-regulated by bone marrow stromal cells from patients with leukemia. Conclusion Meisoindigo can down regulate expression of CXCR4 in leukeimic cells to anti- leukemic cells.

关 键 词：白血病 甲异靛 骨髓基质细胞 CXCR4 

分 类 号：R733.7[医药卫生—肿瘤]